Method for producing sugar solution
US-9212377-B2 · Dec 15, 2015 · US
US9150869B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9150869-B2 |
| Application number | US-201514560749-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 26, 2015 |
| Priority date | Jun 13, 2008 |
| Publication date | Oct 6, 2015 |
| Grant date | Oct 6, 2015 |
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Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode Pichia stipitis proteins that allow recombinant strains of Saccharomyces cerevisiae expressing the protein to grow on xylose, and allow or increase uptake of xylose by Pichia stipitis or Saccharomyces cerevisiae expressing the coding sequences. Expression of the coding sequences enhances uptake of xylose and/or glucose, allowing increased ethanol or xylitol production.
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We claim: 1. A nucleic acid construct comprising a first coding sequence operably linked to a first promoter not natively associated with the coding sequence, the coding sequence encoding a glucose/xylose transporter polypeptide having at least 95% amino acid identity to SEQ ID NO: 2. 2. The nucleic acid construct of claim 1 , wherein the glucose/xylose transporter polypeptide comprises SEQ ID NO: 2. 3. The nucleic acid construct of claim 1…
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