Cell culture transferring instrument

Patent metadata

Field	Value
Publication number	US-9895522-B2
Application number	US-201414309112-A
Country	US
Kind code	B2
Filing date	Jun 19, 2014
Priority date	Dec 20, 2011
Publication date	Feb 20, 2018
Grant date	Feb 20, 2018

Abstract

A cell culture transferring instrument includes a cylindrical body held by an operator not shown in the drawing, a plunger inserted through the interior of the cylindrical body, a plurality of support members connected to a distal end of the plunger, and an adsorbent capable of adsorbing a sheet-shaped cell culture held by the support members. The support members in contact with the distal end of the cylindrical body are elastically deformed by displacing the plunger toward the proximal end side with respect to the cylindrical body in a state in which the sheet-shaped cell culture is adsorbed to the adsorbent, whereby the adsorbent and the sheet-shaped cell culture are deformed and housed in a housing hole in the cylindrical body.

First claim

What is claimed is:

1. A method of transferring a cell culture using a cell culture transferring instrument, the cell culture transferring instrument comprising a hollow body possessing a distal-most end, a displacement member movably positioned in the hollow body, an adsorbent fixed at a distal end of the displacement member, and a supporting member positioned on the adsorbent to maintain a shape of the adsorbent, the method comprising:
axially moving the displacement member towards the cell culture to bring the adsorbent and the cell culture into contact with one another when the distal end of the displacement member extends distally beyond the distal-most end of the hollow body and the adsorbent is outside the hollow body;
axially moving the displacement member and the cell culture attached to the adsorbent to move the cell culture to a position inside the hollow body, the hollow body maintaining the adsorbent and the cell culture in a first shape;
positioning the hollow body adjacent to an affected part of a living body;
axially moving the displacement member and the cell culture attached to the adsorbent to position the cell culture outside the hollow body, the adsorbent and the cell culture taking a second shape different from the first shape;
contacting the affected part of the living body with the cell culture such that the cell culture sticks to the affected part of the living body; and
moving the displacement member away from the cell culture while the cell culture remains stuck to the affected part of the living body.

2. The method according to claim 1 , wherein the axial movement of the displacement member and the cell culture attached to the adsorbent to position the cell culture inside the hollow body causes the cell culture to have a concave-shaped cross-section.

3. The method according to claim 1 , wherein the axial movement of the displacement member and the cell culture attached to the adsorbent to position the cell culture inside the hollow body causes both the adsorbent and the cell culture to have a concave-shaped cross-section.

4. The method according to claim 1 , wherein the adsorbent is a cell culture adsorbing film having flexibility and hygroscopicity.

5. The method according to claim 1 , further comprising: maintaining the adsorbent and the cell culture in a substantially planar shape while axially moving the displacement member and the cell culture towards the inside of the hollow body, the cell culture and the adsorbent being maintained in the substantially planar shape by the supporting member.

Assignees

• Terumo Corp

Inventors

• Yori Kouichirou

Classifications

• A61M37/0069 Primary

  Devices for implanting pellets, e.g. markers or solid medicaments (for introducing of radioactive sources for interstitial radiation therapy, i.e. brachytherapy A61N5/1027) · CPC title

• A61F2/02

  Prostheses implantable into the body · CPC title

• A61M2202/0007

  introduced into the body · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• A61M2202/09

  Body tissue · CPC title