Enzyme-modified soybean product

Patent metadata

Field	Value
Publication number	US-9089154-B2
Application number	US-201013383431-A
Country	US
Kind code	B2
Filing date	Jul 14, 2010
Priority date	Jul 17, 2009
Publication date	Jul 28, 2015
Grant date	Jul 28, 2015

Abstract

The present invention relates to a method of producing a modified soybean product, such as a modified soybean meal, comprising use of a proteolytic enzyme. The invention further relates to use of a proteolytic enzyme in the production of a feed or feed additive product.

First claim

The invention claimed is:

1. A method of producing an enzyme-modified soybean product comprising treating an aqueous slurry of soybean flakes with a microbial proteolytic enzyme having specificity for arginine and/or lysine, wherein the enzyme-modified soybean product has a residual trypsin inhibitor activity which is at least 30% lower than a soybean product produced by the same method without addition of the microbial proteolytic enzyme and wherein the microbial proteolytic enzyme has an identity of at least 95% to amino acids 25-248 of SEQ ID NO: 1.

2. The method of claim 1 , wherein the microbial proteolytic enzyme has an identity of at least 98% to amino acids 25-248 of SEQ ID NO: 1.

3. The method of claim 1 , wherein the microbial proteolytic enzyme comprises the sequence of amino acids 25-248 of SEQ ID NO: 1.

4. The method of claim 1 , further comprising grinding the soybean flakes prior to the treatment with the microbial proteolytic enzyme.

5. The method of claim 1 , wherein at least 50% (w/w) of the soybean flakes to be enzyme treated will be retained on a No. 100 mesh (US standard) screen.

6. The method of claim 1 , wherein the soybean flakes are subjected to defatting by solvent extraction before the enzyme treatment.

7. The method of claim 1 , wherein the treatment is performed at a pH between 6and 7.

8. The method of claim 1 , wherein the aqueous slurry of soybean flakes has a dry matter content of at least 10%.

9. The method of claim 1 , wherein the enzyme-modified soybean product is a feed or feed additive.

10. The method of claim 1 , wherein the soybean flakes to be enzyme treated have not been heated to a temperature of 70° C. or above in the presence of more than 20% (w/w) water.

11. The method of claim 1 , wherein the enzyme-modified soybean product has a residual trypsin inhibitor activity which is at least 40% lower than a soybean product produced by the same method without addition of the microbial proteolytic enzyme.

12. The method of claim 1 , wherein the enzyme-modified soybean product has a residual trypsin inhibitor activity which is at least 50% lower than a soybean product produced by the same method without addition of the microbial proteolytic enzyme.

13. The method of claim 1 , wherein the enzyme-modified soybean product has a residual trypsin inhibitor activity which is at least 60% lower than a soybean product produced by the same method without addition of the microbial proteolytic enzyme.

14. A method of producing an enzyme-modified soybean meal comprising
(a) defatting soybean flakes by solvent extraction;
(b) grinding the defatted soybean flakes to a soybean meal; and
(c) treating an aqueous slurry of the soybean meal with a microbial proteolytic enzyme having specificity for arginine and/or lysine, wherein the microbial proteolytic enzyme has an identity of at least 95% to amino acids 25-248 of SEQ ID NO: 1;

wherein step (b) is performed before or after step (c).

15. The method of claim 14 , wherein the microbial proteolytic enzyme has an identity of at least 98% to amino acids 25-248 of SEQ ID NO: 1.

16. The method of claim 14 , wherein the microbial proteolytic enzyme comprises the sequence of amino acids 25-248 of SEQ ID NO: 1.

Assignees

• Andersen Lene N
• Lynglev Gitte B
• Christensen Lars L H
• Machado Rogerio P
• Novozymes As

Inventors

• Andersen Lene N
• Lynglev Gitte B
• Christensen Lars L H
• Machado Rogerio P

Classifications

• C12Y304/19011

  Gamma-D-glutamyl-meso-diaminopimelate peptidase (3.4.19.11) · CPC title

• A23J3/16

  from soybean · CPC title

• A23J3/346

  of vegetable proteins · CPC title

• A23L11/33 Primary

  using enzymes; Enzymatic transformation of pulses or legumes · CPC title

• A23K10/14

  Pretreatment of feeding-stuffs with enzymes · CPC title