Protein isolate and process for the production thereof

Patent metadata

Field	Value
Publication number	US-11832633-B2
Application number	US-201816764739-A
Country	US
Kind code	B2
Filing date	Nov 14, 2018
Priority date	Nov 15, 2017
Publication date	Dec 5, 2023
Grant date	Dec 5, 2023

Abstract

A process for producing a protein isolate from an oilseed meal, and the isolate thus obtained, said isolate comprising proteins and an amount of 4 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate. The process may comprise the following steps:
a) providing an oilseed meal; b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase; c) separating said solid phase from said slurry, d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, preferably from 2 to 3, to form a mixture said mixture having a liquid phase; e) separating said liquid phase from said mixture formed in step d); f) f1) mixing the separated liquid phase to a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase; and/or f2) mixing the separated liquid to a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05M to 0.5M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase; g) precipitating a solid phase from the liquid of step f) for example by a cooling down step of the mixture to a temperature of 30° C. or less; h) separating said solid precipitate from the liquid of step g) said liquid comprising a water-rich liquid phase and an oil-rich liquid phase; i) separating said water-rich liquid phase from said oil-rich liquid phase, j) subjecting said water-rich liquid phase obtained in step i) to one or several membrane filtration(s) to obtain a protein isolate; and k) optionally, drying said protein isolate to obtain a dry protein isolate.

First claim

The invention claimed is:

1. A process for producing a protein isolate from an oilseed meal, said isolate comprising proteins and an amount of 4 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate, said process comprising the following steps:
a) providing cold-pressed, partially defatted, oilseed meal said oilseed meal being selected from the group consisting of rapeseed, canola, flax, lupine, sunflower, safflower, cotton, mustard and hemp seed meals, and mixtures thereof and said oilseed meal comprising from about 5 wt % to about 20 wt % of oil, by total weight of dry matter of partially defatted meal;
b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase;
c) separating said solid phase from said slurry to obtain a separated solid phase;
d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, to form a mixture said mixture having a liquid phase;
e) separating said liquid phase from said mixture formed in the step d) to obtain a separated liquid phase;
f) f1) mixing the separated liquid phase with a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase;
and/or
f2) mixing the separated liquid phase with a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05 M to 0.5 M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase;
g) precipitating a solid phase from the liquid phase of the step f) to obtain a solid precipitate;
h) separating said solid precipitate from the liquid phase of the step g) said liquid phase comprising a water-rich liquid phase and an oil-rich liquid phase;
i) separating said water-rich liquid phase from said oil-rich liquid phase,
j) subjecting said water-rich liquid phase obtained in the step i) to one or several membrane filtration(s) to obtain a protein isolate; and
k) optionally, drying said protein isolate to obtain a dry protein isolate.

2. The process according to claim 1 , wherein the oilseed meal is a cold-press oilseed meal which has been cold-pressed at a temperature of 85° C. or less.

3. The process according to claim 1 , wherein said salt is sodium chloride.

4. The process according to claim 1 , wherein a ratio of the oilseed meal to the first aqueous solvent ranges from 1:5 to 1:20 (w/w).

5. The process according to claim 1 , wherein any one of the steps c), e), h), i) and/or j) is repeated.

6. The process according to claim 1 , wherein the step j) comprises the following steps:
j1) optionally subjecting the water-rich liquid phase obtained in the step i) to at least one microfiltration step and harvesting a permeate,
j2) subjecting the water-rich liquid phase obtained in the step i) or the permeate of the step j1) to at least one ultrafiltration step, optionally followed by at least one diafiltration step, and harvesting the protein isolate.

7. The process according to claim 1 , wherein the steps a) to e), i) and j) are conducted at room temperature.

8. The process according to claim 1 , comprising mixing said separated solid phase with a second aqueous solvent at a pH ranging from 2 to 3, to form a mixture said mixture having a liquid phase.

9. The process according to claim 1 , wherein said precipitating a solid phase from the liquid phase of the step g) comprises a cooling down step of the mixture to a temperature of 30° C. or less.

10. The process according to claim 1 , wherein the oilseed meal is selected from the group consisting of rapeseed, canola and sunflower seed meals and mixtures thereof.

11. The process according to claim 1 , wherein the oilseed meal is a cold-press oilseed meal which has been cold-pressed at a temperature of 60° C. or less.

12. The process according to claim 1 , wherein a ratio of the oilseed meal to the first aqueous solvent ranges from 1:6 to 1:10 (w/w).

13. The process according to claim 1 , wherein oilseeds are first at least partially dehulled before being transformed into said oilseed meal.

14. The process according to claim 1 , wherein the step f1) and/or the step f2) is carried out at 55° C.±2° C.

15. The process according to claim 1 , wherein no hexane is used to extract oil from the oilseed meal.

16. A process for producing a protein isolate from an oilseed meal, said isolate comprising proteins and an amount of 3 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate, said process comprising the following steps:
i. providing cold-pressed, partially defatted, oilseed meal said oilseed meal being selected from the group consisting of rapeseed, canola, flax, lupine, sunflower, safflower, cotton, mustard and hemp seed meals, and mixtures thereof and said oilseed meal comprising from about 5 wt % to about 20 wt % of oil, by total weight of dry matter of partially defatted meal;
ii. mixing said oilseed meal with an aqueous solvent at a pH ranging from 1 to 3.5 to form a mixture said mixture having a liquid phase;
iii. separating said liquid phase from said mixture formed in the step ii) to obtain a separated liquid phase;
iv. mixing the separated liquid phase with a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase; and/or
v. precipitating a solid phase from the liquid phase of the step iv) by a cooling down step of the mixture to a temperature of 30° C. or less to obtain a solid precipitate;
vi. separating said solid precipitate from the liquid phase of the step v), said liquid phase comprising a water-rich liquid phase and an oil-rich liquid phase;
vii. separating said water-rich liquid phase from said oil-rich liquid phase,
viii. subjecting said water-rich liquid phase obtained in the step vii) to one or several membrane filtration(s) to obtain a protein isolate; and
ix. optionally, drying said protein isolate to obtain a dry protein isolate.

17. The process according to claim 16 , comprising mixing said oilseed meal with an aqueous solvent at a pH ranging from 2 to 3, to form a mixture said mixture having a liquid phase.

18. The process according to claim 16 , wherein oilseeds are first at least partially dehulled before being transformed into said oilseed meal.

19. The process according to claim 16 , wherein the step f1) and/or the step f2) is carried out at 55° C.±2° C.

20. The process according to claim 16 , wherein no hexane is used to extract oil from the oilseed meal.

21. A process for producing a protein isolate from an oilseed meal, said isolate comprising proteins and an amount of 3 wt. % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate, said process comprising the following steps:
A. providing cold-pressed, partially defatted, oilseed meal said oilseed meal being selected from the group consisting of rapeseed, canola, flax, lupine, sunflower, safflower, cotton, mustard and hemp seed meals, and mixtures thereof, and said oilseed meal comprising from about 5 wt % to about 20 wt % of oil, by total weight of dry matter of partially defatted meal;
B. mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a liquid phase;
C. separating said liquid phase from said slurry to obtain a separated liquid phase,
D. adjusting t

Assignees

• Avril
• Univ De Lorraine
• Centre Nat Rech Scient

Inventors

• Defaix Claire
• Kapel Romain
• Galet Olivier

Classifications

• A23J1/142 Primary

  by extracting with organic solvents · CPC title

• A23J1/148

  by treatment involving enzymes or microorganisms (enzymatic hydrolysis of proteins A23J3/34) · CPC title

• C07K1/34

  by filtration, ultrafiltration or reverse osmosis · CPC title

• C07K14/76

  Albumins · CPC title

• C12Y301/03

  Phosphoric monoester hydrolases (3.1.3) · CPC title