Optoelectronic detection system

Patent metadata

Field	Value
Publication number	US-9005989-B2
Application number	US-201113039780-A
Country	US
Kind code	B2
Filing date	Mar 3, 2011
Priority date	Feb 7, 2001
Publication date	Apr 14, 2015
Grant date	Apr 14, 2015

Abstract

The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal.

First claim

What is claimed is:

1. A method for detecting target particles in a liquid sample comprising:
a) adding the liquid sample to a chamber;
b) localizing the target particles within the chamber;
c) adding emitter cells, comprising receptors suitable for interaction with target particles and emitter molecules that emit photons in response to the receptors interacting with target particles, to form a mixture;
d) localizing the emitter cells within a chamber;
e) measuring for photon emission from the cells in the mixture, to thereby detect the presence or absence of the target particles.

2. The method of claim 1 , wherein the emitter cells are B-cells.

3. The method of claim 1 , wherein the measuring for photon emission is performed in a single apparatus and with the sample mixture in a single sample receptacle.

4. The method of claim 1 , wherein a plurality of samples are simultaneously analyzed for a plurality of target particles.

5. The method of claim 1 , wherein the emitter cells are localized in the mixture by applying a centrifugal force.

6. The method of claim 1 , wherein the emitter cells are localized in the mixture by fluid removal.

Assignees

• Harper James Douglas
• Mathews Richard Hart
• Johnson Bernadette
• Petrovick Martha Susan
• Rundell Ann
• Nargi Frances Ellen
• Stephens Timothy
• Mendenhall Linda Marie
• Hollis Mark Alexander
• Young Albert M
• Rider Todd H
• Schwoebel Eric David
• Vian Trina Rae
• Massachusetts Inst Technology

Inventors

• Harper James Douglas
• Mathews Richard Hart
• Johnson Bernadette
• Petrovick Martha Susan
• Rundell Ann
• Nargi Frances Ellen
• Stephens Timothy
• Mendenhall Linda Marie
• Hollis Mark Alexander
• Young Albert M
• Rider Todd H
• Schwoebel Eric David
• Vian Trina Rae

Classifications

• G01N21/07 Primary

  Centrifugal type cuvettes (G01N21/09 takes precedence) · CPC title

• G01N21/76 Primary

  Chemiluminescence; Bioluminescence · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title

• Y10S435/808

  Optical sensing apparatus · CPC title

• Y10S436/805

  Optical property · CPC title