Disk-type microfluidic system and method for measuring deformability of blood cells

Patent metadata

Field	Value
Publication number	US-9383372-B2
Application number	US-201214360675-A
Country	US
Kind code	B2
Filing date	Dec 12, 2012
Priority date	Dec 12, 2011
Publication date	Jul 5, 2016
Grant date	Jul 5, 2016

Abstract

A method for measuring a degree of deformation of blood cells includes: supplying blood to a centrifugal container of a disk; centrifuging the blood in the centrifugal container to blood cells and plasma by rotating the disk and detecting an actual moving distance of the blood cells in the centrifugal container every hour; and calculating a first curve representing the actual moving distance of the blood cells in the centrifugal container every hour and a second curve representing a theoretical moving distance of the blood cells every hour and measuring a degree of deformation of the blood cells by comparing the first curve and the second curve.

First claim

The invention claimed is:

1. A disk-shaped microfluidic system comprising:
a disk including a centrifugal container to which blood is supplied and centrifuging the blood in the centrifugal container to blood cells and plasma;
a photographing unit positioned at the upper side of the disk and photographing inside of the centrifugal container every hour; and
a controller connected with the photographing unit, detecting an actual moving distance of the blood cells in the centrifugal container every hour by using the image photographed by the photographing unit, detecting and displaying a first curve representing the actual moving distance of the blood cells in the centrifugal container every hour and a second curve representing a theoretical moving distance of the blood cells every hour, and measuring a degree of deformation of the blood cells by comparing the first curve and the second curve
wherein the controller calculates the second curve using the following equation and measures the degree of deformation of the blood cells by using at least one of a height difference between the first curve and the second curve and a slope difference between the first curve and the second curve:

π
6

⁢

p
p

⁢

d
p
3

⁢

r
p
″

=

π
6

⁢

(

p
p

-

p
f

)

⁢

d
p
3

⁢

w
2

⁢

r
p

-

3
⁢
π
⁢

⁢

d
p

⁢

r
p
′

⁢

{

u
f

⁡

(

1
+

θ

1
/
3

)

⁢

exp
⁡

[

5
⁢
θ

3
⁢

(

1
-
θ

)

]

}

in the equation, π is a circle constant, p p is the density of the blood, d p is a diameter of the blood cells, r″ p is a moving acceleration of the blood cells, p f is the density of the plasma, w is an angular velocity of the disk, r p is a moving distance of the blood cells, r′ p is a moving speed of the blood cells, u f is the viscosity of the plasma and θ is a volume ratio of the blood cells to the entire volume of the blood.

2. The disk-shaped microfluidic system of claim 1 , wherein the disk further comprises:
a plasma container connected with the centrifugal container; and
a micro-valve connected between the centrifugal container and the plasma container.

Assignees

• Postech Acad Ind Found

Inventors

• Kim Dong Sung
• La Moonwoo

Classifications

• G01N35/00069

  whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk · CPC title

• G01N21/07

  Centrifugal type cuvettes (G01N21/09 takes precedence) · CPC title

• G01N33/80 Primary

  involving blood groups or blood types {or red blood cells (white blood cells G01N33/56972)} · CPC title

• G01N2035/0449

  using centrifugal transport of liquid · CPC title

• G01N33/491 Primary

  by separating the blood components (G01N15/05 takes precedence) · CPC title