Methods and kits for cell release

Patent metadata

Field	Value
Publication number	US-8993322-B2
Application number	US-201313776053-A
Country	US
Kind code	B2
Filing date	Feb 25, 2013
Priority date	Sep 30, 2009
Publication date	Mar 31, 2015
Grant date	Mar 31, 2015

Abstract

Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.

First claim

The invention claimed is:

1. A kit for culturing cells comprising:
a cell culture support;
comprising a substrate, and a multilayer polyelectrolyte coating immobilized on the substrate wherein the multilayer polyelectrolyte coating comprises at least one layer of poly-(di(ethyleneglycol)methylether methacrylate)-co-polyacrylic acid; and

a releasing solution for cell release, wherein the releasing solution comprises DMSO.

2. The kit of claim 1 , further…

Assignees

• Gen Electric

Inventors

• Rubinsztajn Slawomir
• Susarla Prameela
• Smith Reginald Donovan
• Loghin Evelina Roxana

Classifications

• C12N5/0068 Primary

  Chemistry & Metallurgy · mapped topic

• C12N2533/32

  Chemistry & Metallurgy · mapped topic

• C12N2539/10

  Chemistry & Metallurgy · mapped topic

• C12M33/00

  Chemistry & Metallurgy · mapped topic

• C12M23/20

  Chemistry & Metallurgy · mapped topic