Variant LovD polypeptides and their uses
US-9181570-B2 · Nov 10, 2015 · US
US8993296B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993296-B2 |
| Application number | US-201414155557-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 15, 2014 |
| Priority date | Dec 27, 2007 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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An object of the present invention is to provide enzymes associated with equol synthesis, genes coding such enzymes, and a process for producing equol and its intermediates using the enzymes and genes. The present invention provides a dihydrodaidzein synthesizing enzyme, tetrahydrodaidzein synthesizing enzyme, equol synthesizing enzyme, and genes coding these enzymes. The present invention also provides a process for synthesizing dihydrodaidzein, tetrahydrodaidzein, and/or equol using these enzymes.
Opening claim text (preview).
The invention claimed is: 1. An isolated polypeptide comprising an amino acid sequence having at least 80% amino acid identity to the amino acid sequence of SEQ ID NO:13, wherein said isolated polypeptide has an activity of synthesizing equol using tetrahydrodaidzein as a substrate, and wherein said isolated polypeptide does not comprise the amino acid sequence of any of SEQ ID NOs:13-15. 2. A process for producing equol, comprising having the isolated polypepti…
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