D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of D-psicose using same
US-9217166-B2 · Dec 22, 2015 · US
US8986948B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986948-B2 |
| Application number | US-200913001326-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 1, 2009 |
| Priority date | Jul 2, 2008 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans , for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol. The present invention also relates to methods for the production of bioethanol, and to methods for the production of further metabolization products, comprising the metabolization of media containing xylose.
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The invention claimed is: 1. A method for the recombinant expression and production of xylose isomerase or for the conversion of xylose to xylulose by an isolated host cell, wherein said isolated host cell is a yeast cell or a filamentous fungal cell, and said method consists of transforming said host cell with a nucleic acid molecule comprising a nucleic acid sequence that encodes a Clostridium phytofermentans xylose isomerase (XI) and wherein said nucleic acid sequence comprise…
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