L-histidine export protein and method of producing l-histidine using same

Patent metadata

Field	Value
Publication number	US-2025382647-A1
Application number	US-202118037998-A
Country	US
Kind code	A1
Filing date	Dec 23, 2021
Priority date	Dec 24, 2020
Publication date	Dec 18, 2025
Grant date	—

Abstract

Provided are a novel protein having histidine exporting activity. an L-histidine producing microorganism modified to express the protein. and a L-histidine producing method using the microorganism.

First claim

1 . An L-histidine producing microorganism, modified to express a protein having at least 60% sequence homology with SEQ ID NO: 12, SEQ ID NO: 13, or a combination thereof.

2 . The L-histidine producing microorganism according to claim 1 , wherein the protein is a foreign protein.

3 . The L-histidine producing microorganism according to claim 1 , wherein the modification is by introduction of a gene encoding an amino acid sequence having at least 60% sequence homology with SEQ ID NO: 12, SEQ ID NO: 13, or a combination thereof.

4 . The L-histidine producing microorganism according to claim 1 , wherein the protein is represented by an amino acid sequence of SEQ ID NOs: 12 and 13, SEQ ID NOs: 41 and 42, or SEQ ID NOs: 44 and 45.

5 . The L-histidine producing microorganism according to claim 4 , wherein the modification is by introduction of a gene encoding an amino acid sequence of SEQ ID NOs: 12 and 13, SEQ ID NOs: 41 and 42, or SEQ ID NOs: 44 and 45.

6 . The L-histidine producing microorganism according to claim 1 , wherein the microorganism is of the genus Corynebacterium or the genus Escherichia.

7 . The L-histidine producing microorganism according to claim 6 , wherein the microorganism is Corynebacterium glutamicum or Escherichia coli.

8 . A composition for producing L-histidine, comprising
a protein having at least 60% sequence homology with SEQ ID NO: 12, SEQ ID NO: 13, or a combination thereof, a gene encoding the protein, a recombinant vector comprising the gene, or a recombinant microorganism comprising the gene or the recombinant vector.

9 . The composition for producing L-histidine according to claim 8 , wherein the protein is represented by an amino acid sequence of SEQ ID NOs: 12 and 13, SEQ ID NOs: 41 and 42, or SEQ ID NOs: 44 and 45.

10 . The composition for producing L-histidine according to claim 8 , wherein the microorganism is of the genus Corynebacterium or the genus Escherichia.

11 . The composition for producing L-histidine according to claim 10 , wherein the microorganism is Corynebacterium glutamicum or Escherichia coli.

12 . A method for producing L-histidine, comprising culturing the L-histidine producing microorganism according to claim 1 in a medium.

13 . The method for producing L-histidine according to claim 12 , further comprising recovering L-histidine from the cultured microorganism or medium, after the culturing.

14 . The method for producing L-histidine according to claim 12 , wherein the microorganism is of the genus Corynebacterium or the genus Escherichia.

15 . The method for producing L-histidine according to claim 14 , wherein the microorganism is Corynebacterium glutamicum or Escherichia coli.

Assignees

• Cj Cheiljedang Corp

Inventors

• Huh Lan
• Seo Chang Il
• Cheong Ki Yong
• Lim Su-Bin

Classifications

• C12N15/77 Primary

  for Corynebacterium; for Brevibacterium · CPC title

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• C12R2001/19

  Escherichia coli · CPC title

• C12N1/205

  Bacterial isolates · CPC title