Cell culture device and culture vessel

Patent metadata

Field	Value
Publication number	US-2025333681-A1
Application number	US-202519261087-A
Country	US
Kind code	A1
Filing date	Jul 7, 2025
Priority date	Jan 12, 2023
Publication date	Oct 30, 2025
Grant date	—

Abstract

A cell culture device includes: a vessel arrangement stand on which a culture vessel is arranged, the culture vessel being a vessel in which a second cell generated from a first cell is cultured, wherein the vessel arrangement stand includes: a stage on which the culture vessel is arranged; and a magnet, and before culturing the second cell, a liquid that includes the first cell to which a magnetic bead that is an example of a magnetic particle is attached is held in the culture vessel.

First claim

1 . A cell culture device comprising:
a vessel arrangement stand on which a culture vessel is arranged, the culture vessel being a vessel in which a second cell generated from a first cell is cultured, wherein the vessel arrangement stand includes:
a stage on which the culture vessel is arranged; and
a magnet, and
before culturing the second cell, a liquid that includes the first cell to which a magnetic particle is attached is held in the culture vessel.

2 . The cell culture device according to claim 1 , comprising:
a pump that discharges a liquid from the culture vessel, wherein when the pump discharges, from the culture vessel, the liquid that includes the first cell to which the magnetic particle is attached, the magnet applies a magnetic load to the culture vessel to attract the first cell.

3 . The cell culture device according to claim 1 , comprising:
an oscillation mechanism that oscillates the stage to cause the stage to tilt with respect to a horizontal direction, wherein the vessel arrangement stand includes a plurality of magnets each of which is the magnet.

4 . The cell culture device according to claim 3 , wherein
the plurality of magnets are disposed at a plurality of positions in a direction in which the stage tilts.

5 . The cell culture device according to claim 3 , comprising:
a pump that discharges a liquid from the culture vessel, wherein when the pump discharges, from the culture vessel, the liquid that includes the first cell to which the magnetic particle is attached, the first cell to which the magnetic particle is attached is attracted by using, among the plurality of magnets, at least one magnet that is positioned closer to a fulcrum at which the stage tilts.

6 . The cell culture device according to claim 5 , wherein
the plurality of magnets are controlled to attract the second cell by using a greater number of the plurality of magnets than the at least one magnet, the second cell having been cultured.

7 . The cell culture device according to claim 1 , wherein
a heater is provided to the stage.

8 . The cell culture device according to claim 7 , wherein
the magnet and the heater are alternately arranged.

9 . The cell culture device according to claim 8 , wherein
the magnet and the heater are arranged in a stripe pattern.

10 . The cell culture device according to claim 7 , comprising:
a controller that controls output of the heater in accordance with a weight of a liquid in the culture vessel or a tilt of the stage.

11 . The cell culture device according to claim 1 , comprising:
a hold-down plate that holds down, on the stage, the culture vessel arranged on the stage.

12 . The cell culture device according to claim 11 , wherein
the hold-down plate is held by an elastic body.

13 . The cell culture device according to claim 11 , wherein
the hold-down plate is provided at a position that opposes the magnet.

14 . The cell culture device according to claim 13 , wherein the hold-down plate is constituted from a magnetic material.

15 . The cell culture device according to claim 1 , wherein
the magnet is a permanent magnet, and the cell culture device comprises a movement mechanism that is capable of moving the magnet to change a distance between the magnet and the stage.

16 . The cell culture device according to claim 1 , wherein
a culture medium and the liquid that includes the first cell are supplied to the culture vessel, the culture medium being for culturing the second cell.

17 . The cell culture device according to claim 16 , wherein
a supply path and a discharge path are connected to the culture vessel, the supply path being for supplying the culture medium and the liquid that includes the first cell to the culture vessel, the discharge path being for discharging vessel liquid present in the culture vessel, the liquid that includes the first cell is supplied to the culture vessel from a cell vessel, the culture medium is supplied to the culture vessel from a culture medium vessel, and the cell vessel, the culture medium vessel, and the culture vessel define a closed space when the cell vessel and the culture medium vessel are each connected to the supply path and the discharge path.

18 . The cell culture device according to claim 1 , wherein
a liquid including a viral vector is supplied to the culture vessel to infect the first cell with a virus to generate the second cell.

19 . The cell culture device according to claim 1 , wherein
the first cell is a hematopoietic stem cell, the liquid that includes the first cell is blood, and the second cell is an induced pluripotent stem (IPS) cell.

20 . A culture vessel arranged in the cell culture device according to claim 1 .

21 . The culture vessel according to claim 20 , comprising:
a supply port through which a culture medium and the liquid that includes the first cell are supplied to the culture vessel, the culture medium being from a culture medium vessel, the liquid that includes the first cell being from a cell vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.

22 . The culture vessel according to claim 21 , wherein
the cell vessel, the culture medium vessel, and the culture vessel define a closed space when the cell vessel and the culture medium vessel are connected to the supply port.

23 . The culture vessel according to claim 21 , wherein
in a top view, a shape of the culture vessel includes two short sides that oppose each other in one direction, and two long sides that oppose each other in an other direction that intersects the one direction, the culture vessel includes: a first supply port and a second supply port that are each the supply port; and a first discharge port and a second discharge port that are each the discharge port, the first supply port is provided to one of the two short sides of the culture vessel, the first discharge port is provided to an other of the two short sides of the culture vessel, and the second supply port and the second discharge port are provided to the other of the two short sides of the culture vessel, or are separately provided to one side and an other side, respectively, of the two long sides of the culture vessel.

24 . The culture vessel according to claim 21 , wherein
a supply path is connected to the supply port, the supply path being for supplying, to the culture vessel, the culture medium and the liquid that includes the first cell, a discharge path is connected to the discharge port, the discharge path being for discharging the vessel liquid present in the culture vessel, and the supply path and the discharge path are provided separately from each other.

25 . The culture vessel according to claim 24 , wherein
a cross-sectional area of a flow path of the supply path and a cross-sectional area of a flow path of the discharge path are different from each other.

26 . The culture vessel according to claim 24 , wherein
a filter that traps the second cell is provided to the discharge path, the second cell being included in the culture medium discharged from the culture vessel, the supply path includes a culture medium supply path through which the culture medium is supplied to the culture vessel, and the culture medium is

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Okumura Koji
• Nakagawa Tohru
• Usui Yukiya
• Kato Hikaru
• Doi Masateru

Classifications

• C12M23/50

  Means for positioning or orientating the apparatus (C12M41/08 takes precedence) · CPC title

• C12M25/16

  Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00) · CPC title

• C12M35/06 Primary

  Magnetic means (C12M35/02 takes precedence) · CPC title

• C12M41/12

  of temperature (controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00) · CPC title

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title