Cannabis plants exhibiting powdery mildew resistance and methods for obtaining same

Patent metadata

Field	Value
Publication number	US-2025268163-A1
Application number	US-202218282256-A
Country	US
Kind code	A1
Filing date	May 6, 2022
Priority date	May 7, 2021
Publication date	Aug 28, 2025
Grant date	—

Abstract

The present technology is in the field of plant breeding and genetics, particularly as it pertains to the genus Cannabis . More specifically, there are provided methods and compositions for producing a population of Cannabis plants with enhanced resistance to powdery mildew. The methods use the detection of molecular genetic markers linked to powdery mildew resistance loci to select for plants displaying an enhanced powdery mildew resistance phenotype.

First claim

1 . A method for creating a population of Cannabis plants with enhanced powdery mildew resistance, the method comprising: a) providing a first population of Cannabis plants; b) detecting the presence of a genetic marker that is genetically linked to a powdery mildew resistance locus on a defined linkage group by 20 cM or less in the first population; c) selecting one or more Cannabis plants containing said marker from the first population of Cannabis plants; and d) producing a population of offspring from at least one of said selected Cannabis plants.

2 . The method of claim 1 , wherein the genetic marker detected is genetically linked to the powdery mildew resistance locus by less than about 15 cM.

3 . The method of claim 1 , wherein the genetic marker detected is genetically linked to the powdery mildew locus by less than about 10 cM.

4 . The method of claim 1 , wherein the genetic marker detected is genetically linked to the powdery mildew locus by less than about 5 cM.

5 . The method of claim 1 , wherein the genetic marker detected is located within a chromosome interval comprising and flanked by a first marker and a second marker.

6 . The method of claim 5 , wherein the chromosome interval is located on Chromosome 2.

7 . The method of claim 6 , wherein the chromosome interval is located on Chromosome 2 at 83660977-84353662 bp.

8 . The method of claim 1 , wherein the genetic marker detected is located within a chromosome interval comprising and flanked by a first nucleic acid sequence and a second nucleic acid sequence, optionally wherein the chromosome interval is located on Chromosome 2 at 83660977-84353662 bp.

9 . The method of claim 1 , wherein the genetic marker is selected from the markers set forth in FIGS. 4 A- 4 E .

10 . The method of claim 1 , wherein the genetic marker has a sequence selected from the sequences set forth in FIGS. 5 A- 5 M and sequences having at least, greater than or about 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity thereto.

11 . A method of creating a population of Cannabis plants comprising at least one allele associated with enhanced powdery mildew resistance comprising at least one sequence selected from a group of resistance marker nucleic acid sequences, the method comprising the steps of: a) genotyping a first population of Cannabis plants, said population containing at least one allele associated with enhanced powdery mildew resistance, the at least one allele associated with enhanced powdery mildew resistance comprising at least one sequence selected from the group consisting of resistance marker nucleic acid sequences; b) selecting from said first population one or more identified Cannabis plants containing said at least one allele associated with enhanced powdery mildew resistance comprising at least one sequence selected from the group consisting of resistance nucleic acid sequences; and c) producing from said selected Cannabis plants a second population, thereby creating a population of Cannabis plants comprising at least one allele associated with enhanced powdery mildew resistance comprising at least one sequence selected from the group consisting of resistance nucleic acid sequences.

12 . The method of claim 11 , wherein the at least one allele comprises at least one allele set forth in FIGS. 4 A- 4 E .

13 . The method of claim 11 , wherein the at least one sequence is selected from the sequences set forth in FIGS. 5 A- 5 M and sequences having at least, greater than or about 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity thereto.

14 . A method for creating a population of Cannabis plants with enhanced powdery mildew resistance, the method comprising: a) providing a first population of Cannabis plants; b) concurrently detecting the presence of at least one genetic marker that is genetically linked to at least one resistance loci by about 20 cM or less in the first population; c) selecting one or more Cannabis plants containing said at least one marker from the first population of Cannabis plants; and d) producing a population of offspring from at least one of said selected Cannabis plants.

15 . The method of claim 14 , wherein the at least one genetic marker detected is genetically linked to at least one resistance loci by less than about 15 cM.

16 . The method of claim 14 , wherein the at least one genetic marker detected is genetically linked to at least one resistance loci by less than about 10 cM.

17 . The method of claim 14 , wherein the at least one genetic marker detected is genetically linked to at least one resistance loci by less than about 5 cM.

18 . The method of claim 14 , wherein the at least one resistance loci is located on Chromosome 2.

19 . The method of claim 18 , wherein the at least one resistance loci is located within the chromosome interval on Chromosome 2 at 83660977-84353662 bp.

20 . The method of claim 14 , wherein the at least one genetic marker is selected from the markers set forth in FIGS. 4 A- 4 E .

21 .- 56 . (canceled)

Assignees

• Canopy Growth Corp

Inventors

• Deokar Amit

Classifications

• G01N33/56961

  Plant cells or fungi · CPC title

• C12Q2600/13

  Plant traits · CPC title

• A01H5/12

  Leaves · CPC title

• A01H1/1255

  for fungal resistance · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title