Disease resistance alleles in soybean

Patent metadata

Field	Value
Publication number	US-10253379-B1
Application number	US-201715433264-A
Country	US
Kind code	B1
Filing date	Feb 15, 2017
Priority date	Mar 4, 2016
Publication date	Apr 9, 2019
Grant date	Apr 9, 2019

Abstract

Methods for conveying Phytophthora sojae resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing Phytophthora sojae resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with Phytophthora sojae resistance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; soybean plants, seeds, and tissue cultures produced by any of the disclosed methods; seed produced by the disclosed soybean plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons to be used in selecting or identifying Phytophthora sojae resistant soybean lines.

First claim

That which is claimed:

1. A method of producing a phytophthora tolerant soybean plant or part thereof, the method comprising the steps of:
(a) isolating a nucleic acid from a soybean plant or part thereof;
(b) detecting, in said soybean plant or part thereof, the presence of a marker associated with phytophthora tolerance in a soybean plant, wherein said marker is located within
a chromosomal interval on chromosome 13 defined by and including positions 28,635,016 to 30,391,751 comprising a G allele at SY0079A corresponding to position 404 of SEQ ID NO: 1, a G allele at SY2931 corresponding to position 495 of SEQ ID NO: 2, and a deletion at SY0818AQ corresponding to position 499 of SEQ ID NO: 3;

(c) identifying a first soybean plant on the basis of the presence of the marker detected in (b);
(d) crossing the first soybean plant with a second soybean plant not comprising the marker detected in (b); and
(e) producing a progeny plant from the cross of (d), wherein said progeny plant comprises the marker detected in (b), thereby producing a phytophthora tolerant soybean plant or part thereof.

Assignees

• Syngenta Participations Ag

Inventors

• Xu Zhanyou
• Doubler Tracy William
• Meda Anderson Rotter
• Breitinger Becky Welsh
• Yu Ju-Kyung
• Ramasubramaniam Harikrishnan

Classifications

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• A01H5/10

  Seeds · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title