Biocompatible member and method for manufacturing same

Patent metadata

Field	Value
Publication number	US-2024425809-A1
Application number	US-202418828533-A
Country	US
Kind code	A1
Filing date	Sep 9, 2024
Priority date	Mar 16, 2022
Publication date	Dec 26, 2024
Grant date	—

Abstract

A biocompatible member according to an embodiment is a biocompatible member including multiple fibers including a biocompatible material. Mutually adjacent fibers among the fibers are covalently bonded at multiple locations in an extension direction of the fibers.

First claim

What is claimed is:

1 . A biocompatible member, comprising:
a plurality of fibers including a biocompatible material, mutually-adjacent fibers among the fibers being covalently bonded at a plurality of locations in an extension direction of the fibers.

2 . The biocompatible member according to claim 1 , wherein
the fibers extend in a first direction in a first region, the fibers extend in a second direction crossing the first direction in a second region, the first region and the second region are stacked, and at a boundary between the first region and the second region, a plurality of the fibers adjacent to one of the fibers in a stacking direction is covalently bonded to the one of the fibers.

3 . The biocompatible member according to claim 2 , wherein
a compressive elastic modulus of the biocompatible member in the stacking direction is not less than 5 kPa.

4 . The biocompatible member according to claim 2 , wherein
a storage modulus is greater than a loss modulus in a region from room temperature to 90° C. when a viscoelasticity-temperature curve of the biocompatible member is measured by a dynamic viscoelastic technique.

5 . The biocompatible member according to claim 2 , wherein
the first region and the second region are alternately stacked.

6 . The biocompatible member according to claim 1 , wherein
the extension direction of the fibers are uniform in one region.

7 . The biocompatible member according to claim 1 , wherein
atoms in the biocompatible material are directly covalently bonded to each other.

8 . The biocompatible member according to claim 1 , wherein
the covalent bonding is performed by crosslinking, the biocompatible member does not include a chemically crosslinked component, and adjacent fibers among the fibers in the biocompatible member are thermally crosslinked.

9 . The biocompatible member according to claim 1 , wherein
a shape of the biocompatible member is maintained even when immersed in a culture medium for 6 days at 37° C.

10 . The biocompatible member according to claim 1 , further comprising:
a liquid having water as a major component, a content ratio of the liquid being not less than 40 mass % and not more than 90 mass %.

11 . The biocompatible member according to claim 10 , wherein
the liquid is at least one of purified water, a physiologic saline solution, serum, a liquid culture medium, protein, RNA, DNA, an aqueous solution including a substrate, a suspension of a cell, a body fluid, and blood.

12 . The biocompatible member according to claim 1 , wherein
voids having diameters of not less than 10 μm are provided in a surface of the biocompatible member at a density of not less than 1/mm 2 .

13 . The biocompatible member according to claim 1 , wherein
the biocompatible material is collagen.

14 . The biocompatible member according to claim 13 , wherein
the collagen is higher-order collagen having a three-dimensional structure.

15 . The biocompatible member according to claim 14 , wherein
the biocompatible member is transparent or semi-transparent.

16 . The biocompatible member according to claim 1 , wherein
the biocompatible member is sheet-like, and a thickness of the biocompatible member is not less than 0.03 mm and not more than 50 mm.

17 . A method for manufacturing a biocompatible member, comprising:
forming a deposited body by depositing fibers including a biocompatible material; causing a liquid to penetrate the deposited body, the liquid being volatile; volatilizing the liquid that penetrated the deposited body; and performing thermal crosslinking treatment of the deposited body after the liquid is volatilized in an environment decompressed from atmospheric pressure, the forming of the deposited body including making extension directions of the deposited fibers uniform by pulling the fibers in one direction, the penetration of the volatile liquid including reducing a distance between mutually-adjacent fibers among the fibers by a capillary force acting between the mutually-adjacent fibers among the fibers, the thermal crosslinking treatment including covalently bonding the fibers having the reduced distance to each other at a plurality of locations.

18 . The method for manufacturing the biocompatible member according to claim 17 , wherein
the penetration of the volatile liquid includes forming a space in the deposited body not filled with the volatile liquid.

19 . The method for manufacturing the biocompatible member according to claim 17 , further comprising:
performing hydrostatic pressure treatment of the deposited body after the thermal crosslinking treatment.

20 . The method for manufacturing the biocompatible member according to claim 17 , wherein
the thermal crosslinking treatment includes exposing the deposited body after the liquid is volatilized to an atmosphere having a pressure of not more than 10 Pa and a temperature of not less than 100° C. and not more than 200° C.

Assignees

• National Univ Corporation Tokyo Medical And Dental Univeristy
• Toshiba Kk

Inventors

• Kimura Tsuyoshi
• Tokuno Yoko
• Kishida Akio
• Uchida Kenya

Classifications

• C12N2537/10

  Cross-linking · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• A61L27/60

  Materials for use in artificial skin · CPC title

• A61L27/50

  Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} · CPC title