Thermotolerant protein glutaminase

Patent metadata

Field	Value
Publication number	US-2023416718-A1
Application number	US-202118037934-A
Country	US
Kind code	A1
Filing date	Nov 19, 2021
Priority date	Nov 20, 2020
Publication date	Dec 28, 2023
Grant date	—

Abstract

Disclosed is a protein glutaminase having improved thermotolerance. A protein glutaminase comprising a polypeptide indicated in any among (1) to (3) has improved thermotolerance. (1) A polypeptide comprising a SEQ ID NO: 1 or 2 amino acid sequence; (2) a polypeptide wherein one or more amino acid residues are substituted, added, inserted or lost in the amino acid sequence indicated by SEQ ID NO: 1 or 2 and exhibiting the same thermotolerance as the amino acid sequence indicated by SEQ ID NO: 1 or 2: and (3) a polypeptide having at least 76% sequence identity with the amino acid sequence indicated by SEQ ID NO: 1 or 2 and having similar thermotolerance to a polypeptide comprising the amino acid sequence indicated by SEQ ID NO: 1 or 2.

First claim

1 . A protein-glutaminase comprising a polypeptide shown in any one of the following (1) to (3):
(1) a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 or 2; (2) a polypeptide having an amino acid sequence obtained by substituting, adding, inserting, or deleting one or more amino acid residues in an amino acid sequence shown in SEQ ID NO: 1 or 2, the polypeptide having thermostability equivalent to thermostability of a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 or 2; and (3) a polypeptide having an amino acid sequence having a sequence identity to an amino acid sequence shown in SEQ ID NO: 1 or 2 of 76% or more, the polypeptide having thermostability equivalent to thermostability of a polypeptide having an amino acid sequence shown in SEQ ID NO: 1 or 2.

2 . A DNA encoding the protein-glutaminase according to claim 1 .

3 . An expression cassette or a recombinant vector comprising the DNA according to claim 2 .

4 . A transformant obtained by transforming a host with the expression cassette or the recombinant vector according to claim 3 .

5 . A method for producing the protein-glutaminase according to claim 1 , the method comprising a step of culturing the transformant according to claim 4 .

6 . An enzyme preparation comprising the protein-glutaminase according to claim 1 .

7 . A protein modifier comprising the protein-glutaminase according to claim 1 .

8 . A method for producing a modified protein material, the method comprising a step of making the protein-glutaminase according to claim 1 act on a protein material.

9 . The method according to claim 8 , wherein the protein material is edible.

Assignees

• Amano Enzyme Inc

Inventors

• Matsuoka Tomohiro
• Yachi Hiroyuki
• Senda Daiki
• Yuki Kensuke

Classifications

• C12N9/80 Primary

  acting on amide bonds in linear amides {(3.5.1)} · CPC title

• C12Y305/01044

  Protein-glutamine glutaminase (3.5.1.44) · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• A23J3/34

  using enzymes · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title