Methods, apparatus, and systems for an optical fiber forward scatter channel in flow cytometers

Patent metadata

Field	Value
Publication number	US-2023168178-A1
Application number	US-202217994395-A
Country	US
Kind code	A1
Filing date	Nov 28, 2022
Priority date	Nov 30, 2021
Publication date	Jun 1, 2023
Grant date	—

Abstract

An optical fiber forward scatter channel in a flow cytometer is disclosed. A detector system in the flow cytometer includes fiber optic cable for receiving scattered light from an incident laser light that is directed at cells/particles passing through the flow cytometer. The fiber optic cable delivers the scattered light to a sensor system, which collects data to perform analyses on the scattered light. Such analyses may include, for example, calculating the size of a cell/particle, counting cells/particles, and so on. The fiber optic cable is an inherently efficient and accurate filter for the acceptance or rejection of the scattered light.

First claim

What is claimed is:

1 . A method comprising:
flowing a cell/particle in a flow cell through an optical axis of an incident laser light; striking portions of the cell/particle with the incident laser light to generate forward scattered laser light; focusing the forward scattered laser light into a first end of an optical fiber; directing the forward scattered laser light towards a second end of the optical fiber; and launching the forward scattered laser light out of the second end of the optical fiber into a sensor subsystem to detect the forward scattered laser light.

2 . The method of claim 1 , further comprising:
with the optical fiber, filtering out unwanted scattered light from spatial and angular perspectives.

3 . The method of claim 1 , further comprising:
filtering the forward scattered laser light into a pass band of wavelengths of forward scattered laser light; and detecting an intensity of the pass band of wavelengths of forward scattered laser light.

4 . The method of claim 3 , wherein
the pass band of wavelengths of forward scattered laser light is in a range inclusively between 492 nanometers and 484 nanometers around 488 nanometers.

5 . The method of claim 1 , further comprising:
receiving the forward scattered laser light out from the second end of the optical fiber; focally reimaging the forward scattered laser light into a photodiode detector; filtering the focused forward scattered laser light into a pass band of wavelengths of forward scattered laser light into the photodiode detector; and detecting an intensity of the pass band of wavelengths of forward scattered laser light.

6 . The method of claim 5 , further comprising:
adjusting a signal gain of the photodiode detector to detect the intensity of the pass band of wavelengths of the forward scattered laser light.

7 . The method of claim 1 , further comprising:
prior to the striking, blocking the incident laser light.

8 . The method of claim 7 , wherein
the incident laser light is blocked while the cell/particle is outside the optical axis of the incident laser light.

9 . The method of claim 1 , further comprising:
after the striking, blocking the incident laser light, wherein the incident laser light is blocked while the cell/particle is outside the optical axis of the incident laser light.

10 . The method of claim 5 , wherein
the photodiode detector is an avalanche photodiode.

11 . A forward scatter light detector system comprising:
an obscuration device aligned in an optical axis of a laser to block incident laser light that is unscattered by a cell/particle; a first lens having an optical axis aligned with the optical axis of the laser, the first lens spaced apart from an interrogation region in a flow cell of cells/particles by a first distance, the first lens spaced apart from the obscuration device by a second distance less than the first distance, the first lens to received forward scattered laser light that is scattered by the cells/particles that are struck by incident laser light; an optical fiber having a first end and a second end opposite the first end, the optical fiber having an optical axis at the first end aligned with the optical axis of the laser, the first end of the optical fiber spaced apart from the first lens by a third distance; and a sensor subsystem having an optical axis aligned with the optical axis of the optical fiber at the second end, the sensor subsystem to receive forward scattered laser light from the second end of the optical fiber and detect an intensity of the forward scattered laser light.

12 . The forward scatter light detector system of claim 11 , further comprising:
a first mechanical coupler coupled to the optical fiber near the first end to hold the first end such that the optical axis of the optical fiber is aligned with the optical axis of the laser.

13 . The forward scatter light detector system of claim 11 , further comprising:
a second mechanical coupler coupled to the optical fiber near the second end to hold the second end such that the optical axis of the optical fiber is aligned with the optical axis of the sensor subsystem.

14 . The forward scatter light detector system of claim 11 , wherein the sensor subsystem includes
a bandpass filter having a passband range of wavelengths, the bandpass filter having an optical axis aligned with the optical axis of the optical fiber at the second end, the bandpass filter spaced apart from the second end of the optical fiber to receive forward scattered laser light from the second end of the optical fiber, the bandpass filter to filter out scattered light outside the passband range and to pass through the forward scattered laser light within the passband range; and a first photodetector having an optical axis aligned with the optical axis of the optical fiber at the second end, the first photodetector to receive the bandpassed forward scattered laser light to detect an intensity of light therein.

15 . The forward scatter light detector system of claim 11 , wherein the sensor subsystem includes
a second lens having an optical axis aligned with the optical axis of the optical fiber at the second end, the second lens spaced apart from the second end of the optical fiber by a fourth distance to receive forward scattered laser light from the second end of the optical fiber and focally reimage the forward scattered laser light into a focal point; a bandpass filter having a passband range of wavelengths, the bandpass filter having an optical axis aligned with the optical axis of the optical fiber at the second end, the bandpass filter spaced apart from the second lens to receive the reimaged forward scattered laser light from the second lens, the bandpass filter to filter out scattered light outside the passband range and to pass through the forward scattered laser light within the passband range; and an avalanche photodiode detector having an optical axis aligned with the optical axis of the optical fiber at the second end, the avalanche photodiode detector to receive the bandpassed forward scattered laser light to detect an intensity of light therein.

16 . The forward scatter light detector system of claim 15 , wherein
the first distance and the third distance are a focal length of the first lens; and the fourth distance is a focal length of the second lens.

17 . The forward scatter light detector system of claim 11 , wherein
the obscuration device is an obscuration bar.

18 . The forward scatter light detector system of claim 11 , wherein
the obscuration device is an angled mirror to reflect the incident laser light along the optical axis of the laser that is unscattered by a cell/particle.

19 . The forward scatter light detector system of claim 18 , further comprising.
a second photodetector spaced apart from the angled mirror, the second photodetector to receive the reflected incident laser light and sense the intensity of the reflected incident laser light.

20 . The forward scatter light detector system of claim 19 , wherein
an optical axis of the angled mirror has a 45-degree angle with the optical axis of the laser generating the incident laser light such that the incident laser light can be perpendicularly reflected by 90 degrees into the photodetector.

21 . A flow cytometer system comprising:
a platform; a flow cell mounted to the platform; one or more lasers mounted to the platform, the one or more lasers respectively genera

Assignees

• Cytek Biosciences Inc

Inventors

• Shao Qing
• Yan Ming

Classifications

• G01N15/1434 Primary

  Optical arrangements · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N15/1404

  Handling flow, e.g. hydrodynamic focusing · CPC title

• G01N15/149

  specially adapted for sorting particles, e.g. by their size or optical properties · CPC title

• G01N15/01

  specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05) · CPC title