A microfluidic analyser

Patent metadata

Field	Value
Publication number	US-2022355298-A1
Application number	US-202017624483-A
Country	US
Kind code	A1
Filing date	Jul 3, 2020
Priority date	Jul 3, 2019
Publication date	Nov 10, 2022
Grant date	—

Abstract

A microfluidic analyser and a method of using the same is disclosed. The microfluidic analyser comprising a droplet generator, an analyte flow channel in fluid communication with said droplet generator at a first end, wherein said flow channel is configured to allow the droplets to flow in from the first end and exit from a second opposing end, said flow channel receiving at least one illumination channel positioned at a predetermined location between the first and the second end to excite contents of the droplets and said flow channel further comprising a plurality of receiving channels set at predetermined angles to an axis of the flow channel to interrogate at least one optical signal from the illuminated droplet traversing the flow channel and wherein said receiving channels terminate in a signal detector at the distal end away from the flow channel.

First claim

1 . A microfluidic analyser comprising a droplet generator, an analyte flow channel in fluid communication with said droplet generator at a first end, wherein said flow channel is configured to allow the droplets to flow in from the first end and exit from a second opposing end, said flow channel receiving at least one illumination channel positioned at a predetermined location between the first and the second end to excite contents of the droplets and said flow channel further comprising a plurality of receiving channels set at predetermined angles to an axis of the flow channel to interrogate at least one optical signal from the illuminated droplet traversing the flow channel and wherein said receiving channels terminate in a signal detector at the distal end away from the flow channel.

2 . A microfluidic analyser of claim 1 , wherein the droplet generator comprises an analyte inlet fluidically coupled with an analyte source, a buffer inlet fluidically coupled with a buffer source, an analyte inlet channel in fluid communication with the analyte inlet at a first end to receive analyte, a buffer inlet channel in fluid communication with the buffer inlet at a first end to receive buffer, a junction receiving a second end of said analyte channel and a second end of buffer channel, said junction configured to allow interaction of received analyte and buffer, said junction further comprising a constricted fluid path configured to generate droplets to flow into the flow channel.

3 . A microfluidic analyser of claim 1 , wherein at least one illumination channel and receiving channels comprises an optical waveguide.

4 . The microfluidic analyser of claim 3 , wherein at least one waveguide is an optical fibre.

5 . The microfluidic analyser of claim 3 , wherein the waveguide is coupled to an optical source.

6 . The microfluidic analyser of claim 5 , wherein at least one optical fibre comprises a lensed tip.

7 . The microfluidic analyser of claim 1 , further comprising a computing unit capable of receiving at least one optical signal detected by the signal detector and analyzing the received optical signal.

8 . A microfluidic analyser of claim 1 , wherein the droplet generator is coupled to at least one pump to control flow rate in the analyte flow channel.

9 . A microfluidic analyser of claim 1 , wherein the droplet generator is coupled to at least one pump to control droplet size.

10 . A microfluidic analyser of claim 1 , wherein the droplet generator comprises a feedback control configured to optimize flow rate of droplets based on at least one optical signal from an illuminated droplet.

11 . A method using a microfluidic analyser comprising, receiving analyte and buffer by a droplet generator, wherein the analyte and the buffer interact to form at least one droplet flowing into an analyte flow channel, said analyte flow channel in fluid communication with said droplet generator at a first end, wherein said flow channel is configured to allow the droplet to flow in from the first end and exit from a second opposing end, illuminating the said droplet through at least one illumination channel, interrogating optical signals from the illuminated droplet traversing the flow channel through at least one receiving channel, detecting at least one optical signal from the illuminated droplet by at least one signal detector coupled to plurality of receiving channels, analysing the detected optical signal to determine at least one property of contents of the droplet.

12 . The method of claim 11 , wherein a droplet in the analyte flow channel is illuminated by a single optical source through plurality of illuminating channels such that the droplet is subjected to plurality of interrogations by same wavelength of the optical source at plurality of locations, and wherein the interrogations are separated by a time delay based on flow rate of the droplet across the locations.

13 . The method of claim 11 , wherein the optical signals from an illuminated droplet comprises a plurality of optical wavelengths.

14 . The method of claim 11 , wherein flow rate of a droplet is optimized by a feedback control configured to optimize flow rate of the droplet based on at least one optical signal detected from an illuminated droplet.

15 . The method of claim 11 , wherein plurality of optical signals is successively interrogated at plurality of locations traversed by a droplet and the plurality of optical signals from the droplet are analysed by combining the plurality of optical signals into a detector.

16 . The method of claim 15 , wherein flow rate of droplets is optimized to interrogate optical signals from successive droplets such that a droplet is interrogated after a succeeding droplet has traversed all the plurality of locations of interrogation.

17 . The method of claim 11 , wherein the analyte is selected from a group comprising blood cells, single cells from culture cell lines, serum, bacteria, contaminants in liquid, fluorescently labeled cells, beads, microparticles, fluorescently tagged cell organelles, fluorescently tagged nucleic acid probes, and fluorescently tagged nucleic acid proteins.

18 . The method of claims 11 , wherein the buffer is selected from a group comprising oils, surfactants and emulsifiers.

Assignees

• Centre For Cellular And Molecular Platforms
• Indian Institute Of Tech
• Indian Institute Of Tech Madras

Inventors

• Saiyed Taslimarif
• Gupta Preksha
• Prabhakar Anil
• Mohan Ambili

Classifications

• G01N2021/6484

  Optical fibres · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N15/1484 Primary

  microstructural devices · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title

• G01N21/6408

  with measurement of decay time, time resolved fluorescence · CPC title