Method of inducing expression of calcium channel and/or calcium pump, and apparatus therefor

Patent metadata

Field	Value
Publication number	US-2022340872-A1
Application number	US-202217729752-A
Country	US
Kind code	A1
Filing date	Apr 26, 2022
Priority date	Apr 27, 2021
Publication date	Oct 27, 2022
Grant date	—

Abstract

A method of inducing expression of a calcium channel and/or a calcium pump in a cell includes: irradiating the cell with light in a wavelength range of 315-325 nm. The calcium channel and/or the calcium pump is/are at least one selected from the group consisting of dihydropyridine receptor (DHPR), voltage-gated calcium channel (VGCC), ryanodine receptor (RYR), and sarcoendoplasmic reticulum Ca2+-ATPase (SERCA).

First claim

What is claimed is:

1 . A method of inducing expression of a calcium channel and/or a calcium pump in a cell, the method comprising:
irradiating the cell with light in a wavelength range of 315-325 nm, wherein the calcium channel and/or the calcium pump is/are at least one selected from the group consisting of dihydropyridine receptor (DHPR), voltage-gated calcium channel (VGCC), ryanodine receptor (RYR), and sarcoendoplasmic reticulum Ca 2+ -ATPase (SERCA).

2 . The method according to claim 1 , wherein in the step of irradiating, the cell is not irradiated with light at any wavelength of 330-400 nm, or is irradiated with light in wavelengths of 330-400 nm at a fluence that is less than 50% of that of the light in the wavelength range of 315-325 nm.

3 . The method according to claim 1 , wherein in the step of irradiating, the cell is not irradiated with light at any wavelength of 200-300 nm, or is irradiated with light in wavelengths of 200-300 nm at a fluence that is less than 30% of that of the light in the wavelength range of 315-325 nm.

4 . The method according to claim 1 , wherein the light in the wavelength range of 315-325 nm has a wavelength spectrum with a peak wavelength at 320±5 nm and a full width at half maximum in a range of 1-20 nm.

5 . The method according to claim 1 , wherein the light in the wavelength range of 315-325 nm is emitted by a light-emitting diode (LED).

6 . The method according to claim 1 , wherein the cell is an excitatory cell.

7 . A method of modulating calcium signaling in a cell, the method comprising:
inducing expression of a calcium channel and/or a calcium pump in the cell by applying the method according to claim 1 to the cell; and measuring a physiological event involving calcium signaling in the cell.

8 . The method according to claim 7 , wherein the physiological event is one or more selected from the group consisting of muscle contraction, neuronal transmission, cell death, and osteoblast-like differentiation.

9 . A method of making a cell having induced expression of a calcium channel and/or a calcium pump, comprising:
irradiating a cell with light in a wavelength range of 315-325 nm; and freezing the irradiated cell, wherein, in the step of irradiating:
the cell is not irradiated with light at any wavelength of 200-300 nm, or is irradiated with light in wavelengths of 200-300 nm at a fluence that is less than 30% of that of the light in the wavelength range of 315-325 nm, and
the cell is not irradiated with light at any wavelength of 330-400 nm, or is irradiated with light in wavelengths of 330-400 nm at a fluence that is less than 50% of that of the light in the wavelength range of 315-325 nm.

10 . An apparatus for culturing a cell, the apparatus comprising:
a first irradiation unit configured to mainly emit light in a wavelength range of 315-325 nm; a first mounting unit configured to support a cell culture vessel and located within an irradiation area of the first irradiation unit; a first irradiation control unit configured to control the first irradiation unit; and a housing configured to accommodate the first irradiation unit and the first mounting unit therein.

11 . The apparatus according to claim 10 , further comprising:
a second irradiation unit configured to mainly emit light in a wavelength range of 315-325 nm and located below the first mounting unit in the housing; a second mounting unit configured to support a cell culture vessel and located within an irradiation area of the second irradiation unit in the housing; a light blocking member and configured to block light in a wavelength range of 315-325 nm, the light blocking member being located in the housing and configured to prevent the light in the wavelength range of 315-325 nm emitted from the first irradiation unit from being irradiated to the second mounting unit; and a second irradiation control unit configured to control the second irradiation unit.

Assignees

• Nichia Corp

Inventors

• Kawarai Shinpei
• Tsukamoto Atsushi
• Fujikawa Yasuo
• Tsurumoto Tomohiro

Classifications

• C12N5/0062 Primary

  General methods for three-dimensional culture · CPC title

• C12M35/02

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M31/02

  located outside the reactor · CPC title

• C12N2529/10

  Stimulation by light · CPC title

• C12M41/06

  of illumination · CPC title