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Engineering individually addressable cellular spheroids using aqueous two-phase systems
US9176115B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9176115-B2 |
| Application number | US-201314064886-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 28, 2013 |
| Priority date | Oct 26, 2012 |
| Publication date | Nov 3, 2015 |
| Grant date | Nov 3, 2015 |
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Abstract
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Provided is multi-phase systems that may be used to prepare a three-dimension aggregate of cells referred to as a cellular spheroid. The multi-phase system includes a droplet of an aqueous polymer phase within an immersion aqueous polymer phase. The droplet of the droplet aqueous polymer phase contains a three-dimensional aggregate of cells (cellular spheroid). Types of cells that may be used in the multi-phases system include stem cells and cancer cells. The cellular spheroids with the multi-phase system may be used to monitor cell growth in three-dimensional systems, or screen drugs in a three-dimension aggregate of cells.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of producing a cellular spheroid comprising: providing a well containing an immersion aqueous polymer phase; inserting a droplet aqueous polymer phase to form a substantially spherical droplet, where the droplet aqueous polymer phase is immiscible with the immersion aqueous polymer phase; inserting cells selected from cancer and stem cells into the droplet aqueous polymer phase; and allowing the cells to self assemble into a spheroid.…
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Chemistry & Metallurgy · mapped topic
Physics · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
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- What does patent US9176115B2 cover?
- Provided is multi-phase systems that may be used to prepare a three-dimension aggregate of cells referred to as a cellular spheroid. The multi-phase system includes a droplet of an aqueous polymer phase within an immersion aqueous polymer phase. The droplet of the droplet aqueous polymer phase contains a three-dimensional aggregate of cells (cellular spheroid). Types of cells that may be used i…
- Who is the assignee on this patent?
- Tavana Hossein, Atefi Ehsan, Ham Stephanie Lemmo, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification G01N33/5011. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Nov 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).