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Cardiac troponin assay method and assay reagent
US2021215680A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2021215680-A1 |
| Application number | US-201716332647-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 12, 2017 |
| Priority date | Sep 13, 2016 |
| Publication date | Jul 15, 2021 |
| Grant date | — |
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Abstract
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A measurement method and a measurement reagent for cardiac troponin which enable accurate measurement of the amount of cardiac troponin contained in a sample irrespective of the type of the sample and the presence or absence of other components are disclosed. The measurement method for cardiac troponin, wherein cardiac troponin in a sample separated from a body is measured by an immunoassay, includes a pretreatment step of mixing the sample separated from a body with a pretreatment liquid containing one or both of a surfactant and an acidifier. The reagent for immunoassay of cardiac troponin includes a pretreatment liquid containing one or both of a surfactant and an acidifier.
First claim
Opening claim text (preview).
1 . A method of measuring, by immunoassay, cardiac troponin in a sample separated from a body, the method comprising a pretreatment step of mixing the sample separated from a body with a pretreatment liquid containing one or both of a surfactant and an acidifier. 2 . The method according to claim 1 , wherein the pretreatment liquid further contains a reducing agent. 3 . The method according to claim 1 or 2 , wherein the pretreatment liquid contains a surfactant, and the surfactant is one or more selected from the group consisting of anionic surfactants, cationic surfactants, zwitterionic surfactants, and nonionic surfactants. 4 . The method according to claim 3 , wherein the surfactant is an anionic surfactant. 5 . The method according to claim 3 , wherein the pretreatment step is carried out under heat. 6 . The method according to claim 1 , wherein the pretreatment liquid contains an acidifier, and the acidifier has a final concentration of more than 0.05 N and not more than 0.5 N in the pretreatment step. 7 . The method according to claim 1 , wherein the cardiac troponin is cardiac troponin I. 8 . A reagent for immunoassay of cardiac troponin, the reagent comprising a pretreatment liquid containing one or both of a surfactant and an acidifier.
Assignees
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Classifications
- G01N33/5306Primary
Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title
from muscle, cartilage or connective tissue · CPC title
Immunoassay; Biospecific binding assay; Materials therefor · CPC title
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- What does patent US2021215680A1 cover?
- A measurement method and a measurement reagent for cardiac troponin which enable accurate measurement of the amount of cardiac troponin contained in a sample irrespective of the type of the sample and the presence or absence of other components are disclosed. The measurement method for cardiac troponin, wherein cardiac troponin in a sample separated from a body is measured by an immunoassay, in…
- Who is the assignee on this patent?
- Fujirebio Kk
- What technology area does this patent fall under?
- Primary CPC classification G01N33/5306. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Thu Jul 15 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).