Engineered skin equivalent, method of manufacture thereof and products derived therefrom

Patent metadata

Field	Value
Publication number	US-2021122922-A1
Application number	US-202016892839-A
Country	US
Kind code	A1
Filing date	Jun 4, 2020
Priority date	Apr 21, 2016
Publication date	Apr 29, 2021
Grant date	—

Abstract

Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.

First claim

1 .- 198 . (canceled)

199 . A composition comprising: an isolated artificial dermal layer comprising an immortalized keratinocyte cultured in vitro, and a scaffold, wherein the keratinocyte is at least partially in contact with the scaffold.

200 . The composition of claim 199 , wherein at least a portion of a cell layer has been removed.

201 . The composition of claim 199 , wherein the scaffold comprises a natural scaffold, a synthetic scaffold or a combination thereof.

202 . The composition of claim 201 , comprising the synthetic scaffold.

203 . The composition of claim 201 , comprising the natural scaffold.

204 . The composition of claim 199 , wherein the scaffold comprises an at least partially hollow structure.

205 . The composition of claim 199 , wherein the scaffold comprises a collagen, a cellulose, a cotton, an acetate, an acrylic, a latex, a linen, a nylon, a rayon, a velvet, a modacrylic, a saran, a vinyon, a wool, a jute, a hemp, a bamboo, a flax, an alginate, a fibronectin, a poly-p-phenyleneterephthalamide, a polyethylene, a polypropylene, a carrageenan, an agarose, a fibrin, a glass, a silica, an aramid, a carbon, a poly(tetrafluoroethylene), a polyvinyl chloride, a polyvinylidene chloride, a polyvinyl alcohol, a polyacrylonitrile, a chitosan, a polyurethane, a poly(urethane-urea), a polyethylene phthalate, a chitin, a elastin, a keratin, a polyhydroxyalkanoate, a dextran, a pullane, a polyhyaluronic acid, a poly(3-hydroxyalkanoate), a poly(3-hydroxyoctanoate), a poly(3-hydroxyfatty acid), a poly(caprolactone), a poly(para-dioxanone), a laminin, a zein, a casein, a gelatin, a gluten, albumen, a poly L-lactic acid (PLA), a polyglycolic acid (PGA), or any combination thereof.

206 . The composition of claim 199 , wherein the composition further comprises a fibroblast.

207 . The composition of claim 206 , wherein the fibroblast expresses CD10, CD73, CD44, CD90, type I collagen, type III collagen, prolyl-4-hydroxylase beta, or a combination thereof.

208 . The composition of claim 199 , wherein the artificial dermal layer comprises a collagen.

209 . The composition of claim 208 , wherein the collagen is produced at least in part by a collagen producing cell, is separately added, or any combination thereof.

210 . The composition of claim 209 , wherein the collagen producing cell comprises an epithelial cell, a fibroblast, a comeocyte, a Langerhans cell, a basal cell, or a combination thereof.

211 . The composition of claim 210 , wherein the collagen producing cell comprises the epithelial cell wherein the epithelial cell comprises a squamous cell, a cuboidal cell, a columnar cell, a basal cell, or a combination thereof.

212 . The composition of claim 199 , wherein the artificial dermal layer further comprises one or more of keratin, elastin, gelatin, proteoglycan, dermatan sulfate proteoglycan, glycosoaminoglycan, fibronectin, laminin, dermatopontin, lipid, fatty acid, carbohydrate, or a combination thereof.

213 . The composition of claim 199 , wherein a thickness of the artificial dermal layer ranges from about 0.02 mm to about 5 mm.

214 . The composition of claim 199 , wherein the artificial dermal layer further comprises a hair follicle.

215 . The composition of claim 214 , wherein the artificial dermal layer further comprises fur.

216 . The composition of claim 199 , wherein the immortalized keratinocyte is adhered to the scaffold.

217 . The method of claim 199 , wherein the immortalized keratinocyte comprises a collagen producing cell.

218 . The method of claim 199 , wherein the keratinocyte is derived from a mammal, a fish, a bird or a reptile.

219 . The method of claim 218 , comprising the mammal, wherein the mammal comprises an antelope, a bear, a beaver, a bison, a boar, a camel, a caribou, a cat, a cattle, a deer, a dog, an elephant, an elk, a fox, a giraffe, a goat, a hare, a horse, an ibex, a kangaroo, a lion, a llama, a lynx, a mink, a moose, an oxen, a peccary, a pig, a rabbit, a rhino, a seal, a sheep, a squirrel, a tiger, a whale, a wolf, a yak, or a zebra.

220 . The method of claim 218 , comprising the fish, wherein the fish comprises an anchovy, a bass, a catfish, a carp, a cod, an eel, a flounder, a fugu, a grouper, a haddock, a halibut, a herring, a mackerel, a mahi-mahi, a manta ray, a marlin, an orange roughy, a perch, a pike, a pollock, a salmon, a sardine, a shark, a snapper, a sole, a stingray, a swordfish, a tilapia, a trout, a tuna, or a walleye.

221 . The method of claim 218 , comprising the bird, wherein the bird comprises a chicken, a duck, an emu, a goose, a grouse, an ostrich, a pheasant, a pigeon, a quail, or a turkey.

221 . The method of claim 218 , comprising the reptile, wherein the reptile comprises a turtle, a snake, a crocodile, or an alligator.

Assignees

• Vitrolabs Inc
• King S College London

Inventors

• Helgason Ingvar
• Ilic Dusko

Classifications

• C12N2502/13

  connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts" · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12N2501/385

  of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR] · CPC title

• C12N11/02

  Enzymes or microbial cells immobilised on or in an organic carrier · CPC title

• C12N2502/094

  keratinocytes · CPC title