Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses
US-2015374846-A1 · Dec 31, 2015 · US
Agents and methods for treating and preventing seborrheic keratosis
US2017211041A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017211041-A1 |
| Application number | US-201715482899-A |
| Country | US |
| Kind code | A1 |
| Filing date | Apr 10, 2017 |
| Priority date | Apr 26, 2012 |
| Publication date | Jul 27, 2017 |
| Grant date | — |
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Abstract
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Provided herein are methods and assays for isolating and culturing seborrheic keratosis cells ex vivo. Also provided herein are screening assays using cultured seborrheic keratosis cells and methods for treating seborrheic keratosis in a subject.
First claim
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1 . A method for culturing seborrheic keratosis cells ex vivo, the method comprising: (a) contacting a biological sample comprising seborrheic keratosis cells obtained from a subject with a solution comprising a dispase enzyme at a temperature and for a time sufficient to initiate dissociation of the seborrheic keratosis cells from the biological sample, and (b) culturing the dissociated seborrheic keratosis cells. 2 . The method of claim 1 , wherein the temperature is below a standard room temperature of 21° C. 3 . The method of claim 1 , wherein the time sufficient to initiate digestion of the seborrheic keratosis cells is at least 15 hours. 4 . The method of claim 1 , further comprising a step of contacting the biological sample comprising seborrheic keratosis cells with an additional protease. 5 . The method of claim 4 , wherein the additional protease is Trypsin. 6 . The method of claim 1 , further comprising a step of adding a culture medium and filtering larger particles from the dissociated cells before the culturing of step (b). 7 . The method of claim 6 , wherein the dissociated cells are cultured on coated plates.
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- What does patent US2017211041A1 cover?
- Provided herein are methods and assays for isolating and culturing seborrheic keratosis cells ex vivo. Also provided herein are screening assays using cultured seborrheic keratosis cells and methods for treating seborrheic keratosis in a subject.
- Who is the assignee on this patent?
- Massachusetts Gen Hospital
- What technology area does this patent fall under?
- Primary CPC classification C12Y207/11001. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jul 27 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).