Hydrogel-based nanoenulsion for selectively labeling cancer lesion, and preparation method therefor

Patent metadata

Field	Value
Publication number	US-2020384128-A1
Application number	US-201716314624-A
Country	US
Kind code	A1
Filing date	Feb 24, 2017
Priority date	Jun 29, 2016
Publication date	Dec 10, 2020
Grant date	—

Abstract

One embodiment of the present invention relates to a nanoemulsion and a preparation method therefor, the nanoemulsion comprising an oil component, a surfactant, and an aqueous component, wherein the aqueous component comprises a water-soluble active ingredient, a polysaccharide, and hyaluronic acid.

First claim

1 . A nanoemulsion comprising an oil component; a surfactant; and an aqueous component, wherein the aqueous component comprises a water-soluble active ingredient; a polysaccharide; and a hyaluronic acid.

2 . The nanoemulsion of claim 1 , wherein the nanoemulsion comprises an interpenetrating polymer network structure consisting of the water-soluble active ingredient, the polysaccharide, and the hyaluronic acid.

3 . The nanoemulsion of claim 1 , wherein the nanoemulsion has an average particle size of 200 nm or less.

4 . The nanoemulsion of claim 1 , wherein the nanoemulsion is a water-in-oil-type emulsion.

5 . The nanoemulsion of claim 1 , wherein the nanoemulsion comprises 70% by weight to 80% by weight of the oil component, 10% by weight to 20% by weight of the aqueous component, and 5% by weight to 15% by weight of the surfactant, based on the total weight of the nanoemulsion.

6 . The nanoemulsion of claim 1 , wherein the surfactant comprises at least one of a single surfactant or a co-surfactant.

7 . The nanoemulsion of claim 1 , wherein nanoparticles comprised in the nanoemulsion have an average particle size distribution of 0.2 to 0.7 and a zeta potential of −20 mV to −50 mV.

8 . The nanoemulsion of claim 1 , wherein the polysaccharide is alginate.

9 . The nanoemulsion of claim 1 , wherein the water-soluble active ingredient is a water-soluble stain or a water-soluble drug.

10 . The nanoemulsion of claim 9 , wherein the water-soluble stain is any one selected from the group consisting of indigo carmine, methylene blue, Lugol solution, toluidine blue, congo red, phenol red, indocyanine green, fluorescein sodium, and india ink.

11 . The nanoemulsion of claim 9 , wherein a concentration of the water-soluble stain is 1 mM to 10 mM.

12 . The nanoemulsion of claim 1 , wherein a hydrophilic-lipophilic balance value of the nanoemulsion is 6 to 9.

13 . A method of preparing the nanoemulsion of claim 1 , the method comprising:
preparing the oil component; preparing the surfactant; preparing the aqueous component; and mixing and stirring the oil component, the surfactant, and the aqueous component, wherein the aqueous component comprises the water-soluble active ingredient, the polysaccharide, and the hyaluronic acid.

14 . The method of preparing the nanoemulsion of claim 13 , wherein the preparing of the aqueous component comprises preparing a first aqueous component including the polysaccharide and the hyaluronic acid; and preparing a second aqueous component including the water-soluble active ingredient.

15 . The method of preparing the nanoemulsion of claim 14 , wherein a weight ratio of the first aqueous component to the second aqueous component is 1:0.5 to 1:2.

Assignees

• Seoul Nat Univ R&Db Foundation

Inventors

• Lee Kang Won
• Jeong Yoon
• Lee Sara

Classifications

• G01N33/5759

  involving compounds localised on the membrane of tumour or cancer cells · CPC title

• A61K49/00

  Preparations for testing in vivo · CPC title

• G01N33/58 Primary

  involving labelled substances (G01N33/53 takes precedence) · CPC title

• A61K49/006

  Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery · CPC title

• A61K9/0014

  Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00) · CPC title