Solid Phase Extraction Method Using Micro Device

Patent metadata

Field	Value
Publication number	US-2020376410-A1
Application number	US-201916767830-A
Country	US
Kind code	A1
Filing date	Aug 7, 2019
Priority date	Aug 21, 2018
Publication date	Dec 3, 2020
Grant date	—

Abstract

The present invention provides a solid phase extraction method using a micro device having a dam forming portion including a dam, the solid phase extraction method comprising the steps of: (i) injecting a solvent and a filler into the micro device, moving the solvent to the dam forming portion, the dam allowing the solvent to flow therethrough and preventing the filler from passing therethrough, and adsorbing a material to be separated onto the filler in the dam forming portion; and (ii) extracting, from the filler, the adsorbed material, wherein the micro device is rotated with respect to a central axis during one of steps (i) and (ii), and the rotation of the micro device is performed at an angular velocity defined by equation 1.

First claim

1 . A solid phase extraction method using a micro device having a dam-forming portion including a dam, the method comprising the steps of:
(i) injecting a solvent and a filler into the micro device, such that the solvent and the filler move to the dam-forming portion, the dam allowing the solvent to flow therethrough but not allowing the filler to pass therethrough, thereby adsorbing a material to be separated onto the filler in the dam-forming portion; and (ii) extracting the adsorbed material from the filler, wherein during one step of the steps (i) and (ii), the micro device is rotated about a central axis, and the rotation of the micro device is performed at an angular velocity defined by Equation 1 below.

ω
>

g
r



cos


(

90

°

-
ϕ

)

[

Equation



1

]

wherein,
ω is a rotational angular velocity of the micro device,
g is an acceleration of gravity,
r is a radius of the micro device,
φ is an orientation of the micro device, and is in the range of 0<φ<90.

2 . The solid phase extraction method according to claim 1 , wherein the micro device comprises an inlet through which the solvent and the filler are injected; and an outlet through which the solvent is discharged, the dam-forming portion being located between the inlet and the outlet,
wherein each of the dam-forming portion and the dam has a circular cross section with respect to a central axis of the micro device, the central axis extending in a longitudinal direction of the inlet in which the inlet extends, each cross section being perpendicular to the central axis, and wherein the filler is deposited onto the dam in the form of a disk that is centered with respect to the central axis in the dam-forming portion.

3 . The solid phase extraction method according to claim 1 , wherein the rotational angular velocity of the micro device for solid phase extraction is defined in Equation 1-1 below:

ω
>

g

r
dam



cos


(

90

°

-
ϕ

)

[

Equation



1


-


1

]

wherein,
ω is a rotational angular velocity of the micro device,
g is an acceleration of gravity,
r dam is a radius of the dam included in the micro device,
φ is an orientation of the micro device, and is in the range of 0<φ<90.

4 . The solid phase extraction method according to claim 2 ,
wherein each of the inlet and the outlet has a circular cross section with respect to the central axis, the cross section of each of the inlet and the outlet being perpendicular to the central axis, and wherein a diameter of the inlet and a diameter of the outlet are each smaller than a diameter of the dam-forming portion.

5 . The solid phase extraction method according to claim 2 , wherein a rear end portion of the dam which is a surface of the dam facing the inlet has a conical shape protruding toward the inlet.

6 . The solid phase extraction method according to claim 2 , wherein the dam-forming portion has a first end portion connected to the inlet and a second end portion connected to the outlet, the first end portion and the second end portion being opposite ends of the dam-forming portion, the dam is located closer to the second end portion than the first end portion, and the dam spaced apart from the second end portion by a predetermined distance.

7 . The solid phase extraction method according to claim 6 , wherein each of the second end portion and a portion of the dam facing the second end portion has a respective surface protruding toward the outlet.

8 . The solid phase extraction method according to claim 7 , wherein each of the second end portion and a portion of the dam facing the second end portion has a conical shape.

9 . The solid phase extraction method according to claim 1 , wherein the filler is in a form of beads.

10 . The solid phase extraction method according to claim 1 ,
wherein the micro device is rotated by a motor, wherein the motor includes a driving unit for providing rotational power, a rotation shaft connected to the driving unit, and a head connected to the rotation shaft, and wherein the rotation of the micro device is driven by contact between an outer surface of the micro device and the head of the motor.

Assignees

• Lg Chemical Ltd

Inventors

• Choi Junwon
• Kim Dae Hun
• Jegal Seonyoung
• Im Ye Hoon
• Youn Yeu Young

Classifications

• G01N1/405

  by adsorption or absorption · CPC title

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01D11/02 Primary

  of solids · CPC title