Methods and compositions for attenuating allergenicity in protein products

Patent metadata

Field	Value
Publication number	US-2017333386-A1
Application number	US-201515524087-A
Country	US
Kind code	A1
Filing date	Nov 5, 2015
Priority date	Nov 7, 2014
Publication date	Nov 23, 2017
Grant date	—

Abstract

The invention relates to a low sugar or sugar free concentrated polyphenolic extract comprising at least about 30% polyphenols (w/w) and a protein-polyphenol aggregate matrix comprising at least about 15% polyphenols (w/w). The invention further relates to methods of producing a low sugar or sugar free concentrated polyphenolic extract comprising (a) extracting a low sugar or sugar free plant tissue with an aqueous solvent to produce an extract having an aqueous portion and a solids portion; (b) filtering the extract to separate the aqueous portion from the solids portion; and (c) reducing the volume of the separated aqueous portion, and methods of producing a protein-polyphenol aggregate matrix comprising about 1% to about 40% polyphenols (w/w) using the low sugar or sugar free concentrated polyphenolic extract of the invention.

First claim

1 . A low sugar or sugar free concentrated polyphenolic extract comprising at least about 30% polyphenols (w/w).

2 . A protein-polyphenol aggregate matrix comprising at least about 15% polyphenols (w/w).

3 . The protein-polyphenol aggregate matrix of claim 2 , further comprising protein from peanut, tree nut, milk, whey, egg, soy, fish, shellfish, rice, wheat, or any combination thereof.

4 . The method of claim 2 , wherein the protein-polyphenol aggregate matrix is an immunotherapeutic protein product comprising at least about 30% polyphenols (w/w).

5 . A method of producing a low sugar or sugar free concentrated polyphenolic extract, comprising:
(a) extracting a low sugar or sugar free plant tissue with an aqueous solvent to produce a mixture having an aqueous portion and a solids portion; (b) filtering the mixture to separate the aqueous portion from the solids portion; and (c) reducing the volume of the separated aqueous portion to produce a low sugar or sugar free concentrated polyphenolic extract.

6 . The method of claim 5 , wherein the low sugar or sugar free plant tissue comprises a pomace and/or a fermented fruit product.

7 . The method of claim 6 , wherein the pomace is from apple, pomegranate, black currant, blueberry, cranberry, lingnonberry, cherry, grape, muscadine, blackberry, aronia , cinnamon, Sorbaronia mitschurinii, Camellia spp. (tea), peanut (e.g., peanut skins), and/or any combination thereof.

8 . The method of claim 6 , wherein the fermented fruit product is from black currant, blueberry, cranberry, lingnonberry, cherry, grape, muscadine, blackberry, green tea ( Camellia spp.), cinnamon, aronia, Sorbaronia mitschurinii , peanut (e.g., peanut skins) and/or any combination thereof.

9 . The method of claim 5 , wherein the aqueous solvent is water or food grade alcohol at a concentration of about 1% to about 70% (v/v).

10 - 11 . (canceled)

12 . The method of claim 5 , wherein the ratio of low sugar or sugar free plant tissue to aqueous solvent is at least about 1:5 w/v.

13 . The method of claim 5 , wherein the extracting further comprises heating the low sugar or sugar free plant tissue and aqueous solvent.

14 . The method of claim 13 , wherein heating is for about 15 minutes to about 5 hours.

15 . The method of claim 13 , wherein heating comprises a temperature of less than about 80° C.

16 . The method of claim 5 , further comprising:
(d) contacting the low sugar or sugar free concentrated polyphenolic extract with a resin; (e) washing the resin with water; (f) eluting the polyphenols from the resin with food grade alcohol to produce an alcohol polyphenol extract; and (g) evaporating the alcohol in the alcohol polyphenol extract to produce a concentrated polyphenol extract.

17 - 18 . (canceled)

19 . The method of claim 5 , wherein the low sugar or sugar free concentrated polyphenolic extract is contacted with a polyphenol oxidase.

20 . The method of claim 5 , wherein the pH of the low sugar or sugar free concentrated polyphenolic extract is adjusted to about pH 4 to about pH 5.

21 . The method of claim 20 , wherein prior to adjusting the pH, the low sugar or sugar free concentrated polyphenolic extract is contacted with a polyphenol oxidase.

22 . (canceled)

23 . A method of producing a protein-polyphenol aggregate matrix comprising about 1% to about 40% polyphenols, the method comprising:
contacting the low sugar or sugar free concentrated polyphenol extract of claim 1 with a protein product to produce a protein-polyphenol complexed product; and dehydrating the protein-polyphenol complexed product to produce a protein-polyphenol aggregate matrix.

24 . The method of claim 23 , wherein the dehydrating is simultaneous with contacting.

25 . (canceled)

26 . The method of claim 23 , wherein dehydrating the protein-polyphenol aggregate matrix produces a dry granular aggregate.

27 . The method of claim 23 , wherein the concentration of polyphenols in the protein-polyphenol aggregate matrix is about 15 percent to about 75 percent of the weight of the total aggregate.

28 - 35 . (canceled)

36 . A method of attenuating an allergic response in a subject in need thereof, comprising administering to the subject the protein-polyphenol aggregate matrix of claim 2 , wherein the protein polyphenol aggregate matrix comprises about 5% to about 40% polyphenols (w/w).

37 - 45 . (canceled)

Assignees

• Univ North Carolina State

Inventors

• Lila Mary Ann
• Grace Mary H

Classifications

• A61K31/35 Primary

  having six-membered rings with one oxygen as the only ring hetero atom · CPC title

• A61K39/35

  Allergens · CPC title

• C07D311/62

  with oxygen atoms directly attached in position 3, e.g. anthocyanidins · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A23L33/105

  Plant extracts, their artificial duplicates or their derivatives · CPC title