Plants having increased oil quality

Patent metadata

Field	Value
Publication number	US-2020308596-A1
Application number	US-202016831145-A
Country	US
Kind code	A1
Filing date	Mar 26, 2020
Priority date	Mar 28, 2019
Publication date	Oct 1, 2020
Grant date	—

Abstract

This document provides oilseed plants (e.g., pennycress plants) having increased levels of one or more saturated fatty acids, increased levels of one or more polyunsaturated fatty acids (PUFAs), altered (e.g., increased or decreased) levels of oleic acid, and/or altered (e.g., increased or decreased) levels of erucic acid. For example, oilseed plants having reduced polypeptide levels and/or reduced polypeptide activity of one or more polypeptides involved in triglyceride synthesis (e.g., diacylglycerol O-acyltransferase 1 (TAG1) can have increased levels of stearic acid, increased levels of one or more PUFAs, altered levels of oleic acid, and/or altered levels of erucic acid. Also provided herein are methods and materials for making and using oilseed plants having increased levels of one or more saturated fatty acids, increased levels of one or more PUFAs, altered levels of oleic acid, and/or altered levels of erucic acid.

First claim

What is claimed is:

1 . An oilseed plant having a decreased level of erucic acid, as compared to a corresponding wild type oilseed plant, wherein said oilseed plant comprises a modified nucleic acid encoding a TAG1 polypeptide, and wherein said modified TAG1 polypeptide has a reduced expression level or reduced polypeptide activity.

2 . The oilseed plant of claim 1 , wherein said oilseed plant produces oil having from 0 mole % to about 35 mole % erucic acid in the total fatty acid content of the oil.

3 . The oilseed plant of claim 1 , wherein said modified TAG1 polypeptide is selected from the group consisting of a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:3, a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:4, and a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:5.

4 . The oilseed plant of claim 1 , wherein said oilseed plant is selected from the group consisting of pennycress, rapeseed, soybean, sunflower, peanut, canola, flax, camelina, carinata, crambe, and lepidium plants.

5 . A seed produced by the oilseed plant of claim 1 .

6 . Seed oil obtained from the oilseed plant of claim 1 .

7 . An oilseed plant having an increased level of a saturated fatty acid, as compared to a corresponding wild type oilseed plant, wherein said oilseed plant comprises:
a) a modified nucleic acid encoding a TAG1 polypeptide, wherein said modified nucleic acid encoding a TAG1 polypeptide can encode a modified TAG1 polypeptide, wherein said modified TAG1 polypeptide has a reduced expression level or reduced polypeptide activity; and b) a modified nucleic acid encoding a FAD2 polypeptide, wherein said modified nucleic acid encoding a FAD2 polypeptide can encode a modified FAD2 polypeptide, and wherein said modified FAD2 polypeptide has a reduced expression levels or reduced polypeptide activity.

8 . The oilseed plant of claim 7 , wherein said oilseed plant produces oil having from 5 mole % to 55 mole % of said saturated fatty acid in the total fatty acid content of the oil.

9 . The oilseed plant of claim 7 , wherein said saturated fatty acid is stearic acid or palmitic acid.

10 . The oilseed plant of claim 7 , wherein said modified TAG1 polypeptide is selected from the group consisting of a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:3, a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:4, and a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:5.

11 . The oilseed plant of claim 7 , wherein said oilseed plant is selected from the group consisting of pennycress, rapeseed, soybean, sunflower, peanut, canola, flax, camelina, carinata, crambe, and lepidium plants.

12 . A seed produced by the oilseed plant of claim 7 .

13 . Seed oil obtained from the oilseed plant of claim 7 .

14 . An oilseed plant having an increased level of a polyunsaturated fatty acid, as compared to a corresponding wild type oilseed plant, wherein said oilseed plant comprises:
a) a modified nucleic acid encoding a TAG1 polypeptide, wherein said modified nucleic acid encoding a TAG1 polypeptide can encode a modified TAG1 polypeptide, wherein said modified TAG1 polypeptide has a reduced expression level or reduced polypeptide activity; and wherein said oilseed plant comprises: i) a modified nucleic acid encoding a FAE1 polypeptide, wherein said modified nucleic acid encoding a FAE1 polypeptide can encode a modified FAE1 polypeptide, and wherein said modified FAE1 polypeptide has a reduced expression levels or reduced polypeptide activity; or ii) a modified nucleic acid encoding a ROD1 polypeptide, wherein said modified nucleic acid encoding a ROD1 polypeptide can encode a modified ROD1 polypeptide, and wherein said modified ROD1 polypeptide has a reduced expression levels or reduced polypeptide activity.

15 . The oilseed plant of claim 14 , wherein said polyunsaturated fatty acid is linolenic acid, and wherein said oilseed plant produces oil having from 22 mole % to 35 mole % of said linolenic acid.

16 . The oilseed plant of claim 14 , wherein said polyunsaturated fatty acid is linoleic acid, and wherein said oilseed plant produces oil having from 30 mole % to 45 mole % of said linoleic acid.

17 . The oilseed plant of claim 14 , wherein said modified TAG1 polypeptide is selected from the group consisting of a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:3, a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:4, and a modified TAG1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:5

18 . The oilseed plant of claim 14 , wherein said oilseed plant is selected from the group consisting of pennycress, rapeseed, soybean, sunflower, peanut, canola, flax, camelina, carinata, crambe, and lepidium plants.

19 . A seed produced by the oilseed plant of claim 14 .

20 . Seed oil obtained from the oilseed plant of claim 14 .

Assignees

• Univ Minnesota

Inventors

• Marks Michael David
• Chopra Ratan

Classifications

• A01H6/20

  Brassicaceae, e.g. canola, broccoli or rucola · CPC title

• C12N9/1029 Primary

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• A01H5/10

  Seeds · CPC title

• C12N9/001

  acting on the CH-CH group of donors (1.3) · CPC title

• A01H1/06

  Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82}) · CPC title