Process for producing an azaphilone in talaromyces atroroseus

Patent metadata

Field	Value
Publication number	US-2020165645-A1
Application number	US-201816611066-A
Country	US
Kind code	A1
Filing date	May 8, 2018
Priority date	May 8, 2017
Publication date	May 28, 2020
Grant date	—

Abstract

The invention provides a novel class of natural azaphilone pigments, called atrorosins, and methods for their production. The methods for the production of atrorosins include production by fermentation using a fungal species belonging to the genus Talaromyces, preferably the species Talaromycesatroroseus. The use of the novel atrorosins pigments as a colouring agent for food items and/or non-food items, and for cosmetics.

First claim

1 . A method for producing an atrorosin pigment by fermentation, comprising the steps of:
a. providing spores or mycelia of a species of the genus Talaromyces, b. cultivating the spores or mycelia of (a) in a liquid growth medium, c. recovering the atrorosin pigment produced during said cultivating in step b), and d. optionally isolating said atrorosin pigment,
wherein the pH of the growth medium in step (b) is maintained between 4 and 6;
wherein the sole nitrogen source in said liquid growth medium in step (b) is one compound selected from the group consisting of a single amino acid, a peptide, an amino sugar and a primary amine, and
wherein the atrorosin pigment has the structure of Formula I

wherein N—R is selected from the group consisting of an amino acid, a peptide, an amino sugar and a primary amine, and the configuration of the double bond between carbon 2 and 3 is cis.

2 . The method for producing an atrorosin pigment by fermentation according to claim 1 , comprising the additional step of:
a′) cultivating the spores or mycelia of (a) in a preliminary liquid growth medium, wherein the sole nitrogen source is an inorganic nitrogen source and the concentration of NO 3 − is no more than 20 mM, continuing cultivation till the concentration of NO 3 − is depleted to less than 5 mM; and wherein said step (a′) is followed by step (b).

3 . The method according to claim 2 , wherein the sole nitrogen source in step (a′) is an inorganic nitrogen source selected from the group consisting of KNO 3 and NaNO 3 .

4 . The method according to any one of claims 1 to 3 , wherein the sole nitrogen source in step (b) is a single amino acid, selected from the group consisting of: L-alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine, L-glutamine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-serine, L-threonine, L-tyrosine, L-valine and L-ornithine.

5 . The method according to any one of claims 1 to 4 , wherein the species is Talaromyces atroroseus.

6 . The method according to any one of claims 1 to 5 , wherein the preliminary liquid growth medium and the liquid growth medium are synthetic, and comprise salts, trace metals and a carbon source, wherein the salts are KH 2 PO 4 , NaCl, MgSO 4 .7H 2 O, KCl, and CaCl 2 .H 2 O and the trace metals are CuSO 4 .5 H 2 O, Na 2 B 4 O 7 .10 H 2 O, FeSO 4 .7 H 2 O, MnSO 4 .H 2 O, Na 2 MoO 4 .2 H 2 O, and ZnSO 4 .7 H 2 O.

7 . The method according to claim 6 , wherein the carbon source is selected from among glucose, sucrose, maltose, soluble starch, beet or cane molasses, malt, and any combination of at least two thereof.

8 . The method according to any one of claims 1 to 7 , wherein fermentation is performed using batch or fed batch fermentation under aerobic conditions.

9 . The method according to any one of claims 1 to 8 , wherein the liquid growth medium in step (b) is maintained within a pH of 4.0 to 5.5.

10 . An atrorosin pigment having the structure of Formula I,

wherein N—R is selected from among an amino acid, a peptide, an amino sugar and a primary amine, and the configuration of the double bond between carbon 2 and 3 is cis, and wherein the amino acid is selected from the group consisting of: L-alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-serine, L-threonine, L-tyrosine, L-valine and L-ornithine.

11 . The atrorosin pigment according to claim 10 , produced by the method of any one of claims 1 to 8 .

12 . The use of an atrorosin pigment according to claim 10 or 11 as a colouring agent for any one of a food, a non-food product and a cosmetic.

13 . A product comprising the atrorosin pigment according to claim 10 or 11 , wherein the product is selected from among a food, a non-food product and a cosmetic.

14 . A kit for coloring a product, wherein the kit comprises at least one atrorosin pigment according to claim 9 or 10 , wherein the pigment is supplied in a container, wherein the product is selected from among a food, a non-food product and a cosmetic.

Assignees

• Univ Danmarks Tekniske

Inventors

• Tolborg Gerit
• Petersen Thomas Isbrandt
• Larsen Thomas Ostenfeld
• Workman Mhairi

Classifications

• C12P17/188 Primary

  Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms (ergot-alcaloids C12P17/183) · CPC title

• C07D491/048

  the oxygen-containing ring being five-membered · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• A61K8/49

  containing heterocyclic compounds · CPC title

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title