Cell trapping filter, cell trapping device, cell trapping method, cell observation method, and cell culturing method

Patent metadata

Field	Value
Publication number	US-2019338234-A1
Application number	US-201716074237-A
Country	US
Kind code	A1
Filing date	Jan 26, 2017
Priority date	Feb 1, 2016
Publication date	Nov 7, 2019
Grant date	—

Abstract

The filter 105 used in a cell trapping device includes a sheet-like body portion (base metal plating layer 5) containing nickel or copper as a main component and provided with a plurality of through-holes in the thickness direction; a palladium layer 7 containing palladium as a main component and covering the surface of the body portion; and a gold layer 8 containing gold as a main component and covering the surface of the palladium layer.

First claim

1 . A cell trapping filter comprising:
a sheet-like body portion containing nickel or copper as a main component and provided with a plurality of through-holes in a thickness direction; a palladium layer containing palladium as a main component and covering a surface of the body portion; and a gold layer containing gold as a main component and covering a surface of the palladium layer.

2 . The cell trapping filter according to claim 1 , wherein the palladium layer has a thickness of 0.1 to 1 μm.

3 . The cell trapping filter according to claim 1 , further comprising:
a hydrophobic layer formed on a surface of the gold layer by a surface treatment agent having phospholipid in part of a skeleton.

4 . A cell trapping device comprising:
a housing including an introduction flow channel for introducing a sample solution into the inside and a discharge flow channel for discharging the sample solution to the outside; and a cell trapping filter according to claim 1 disposed on a flow channel inside the housing between the introduction flow channel and the discharge flow channel in such a manner that the sample passes through the through-holes.

5 . A cell trapping method comprising selectively trapping a specific cell contained in a sample solution by performing filtration with a cell trapping filter according to claim 1 .

6 . A cell observation method comprising selectively trapping a specific cell contained in a sample solution by performing filtration with a cell trapping filter according to claim 1 , fixing the specific cell trapped on the cell trapping filter with a fixing solution, and then observing the cell under an electron microscope.

7 . A cell culturing method comprising selectively trapping a specific cell contained in a sample solution by performing filtration with a cell trapping filter according to claim 1 and culturing the specific cell trapped on the cell trapping filter in a culture medium.

Assignees

• Hitachi Chemical Co Ltd

Inventors

• Itou Akiko
• Kanbara Hisashige
• Endou Katsuya
• Takahashi Akio

Classifications

• G01N33/56966

  Animal cells · CPC title

• C23C18/1648

  Porous product · CPC title

• C23C18/1834

  Use of organic or inorganic compounds other than metals, e.g. activation, sensitisation with polymers · CPC title

• C23C18/1605

  by masking · CPC title

• C23C18/1644

  porous substrates · CPC title