Method for producing urolithins

Patent metadata

Field	Value
Publication number	US-2019323045-A1
Application number	US-201716473385-A
Country	US
Kind code	A1
Filing date	Dec 26, 2017
Priority date	Dec 26, 2016
Publication date	Oct 24, 2019
Grant date	—

Abstract

An object of the present invention is to provide a method for eliminating the hydroxyl group at the 9-position of a urolithin to produce another kind of urolithin. This object is achieved by a method for producing a second urolithin, including Step (a): allowing, in a solution containing a first urolithin, a microorganism having an ability to produce the second urolithin from the first urolithin to produce the second urolithin from the first urolithin.

First claim

1 - 22 . (canceled)

23 . A method for producing a second urolithin represented by the following General Formula (2), comprising the following Step (a):
Step (a): allowing, in a solution containing a first urolithin represented by the following General Formula (1), a microorganism having an ability to produce the second urolithin represented by the following General Formula (2) from the first urolithin to produce the second urolithin from the first urolithin

(wherein R 1 to R 7 each represent a hydroxyl group, a hydrogen atom, or a methoxy group, and at least one of R 1 to R 7 represents a hydroxy group)

(wherein R 1 to R 7 are identical to the R 1 to R 7 , respectively, of the first urolithin represented by the General Formula (1)).

24 . The production method according to claim 23 ,
wherein the combination of the first urolithin and the second urolithin is a combination of urolithin M5 and urolithin E, respectively, a combination of urolithin M6 and urolithin M7, respectively, a combination of urolithin C and urolithin A, respectively, or a combination of isourolithin A and urolithin B, respectively.

25 . The production method according to claim 23 ,
wherein the microorganism is a microorganism belonging to the genus Clostridium.

26 . The production method according to claim 25 ,
wherein the microorganism belonging to the genus Clostridium is at least one selected from the group consisting of a microorganism belonging to Clostridium bolteae , a microorganism belonging to Clostridium asparagiforme , and a microorganism belonging to Clostridium citroniae.

27 . The production method according to claim 26 ,
wherein the microorganism belonging to Clostridium bolteae is at least one selected from the group consisting of the Clostridium bolteae JCM 12243 strain, DSM 15670 strain, and DSM 29485 strain.

28 . The production method according to claim 26 ,
wherein the microorganism belonging to Clostridium asparagiforme is the Clostridium asparagiforme DSM 15981 strain.

29 . The production method according to claim 26 ,
wherein the microorganism belonging to Clostridium citroniae is the Clostridium citroniae DSM 19261 strain.

30 . The production method according to claim 24 ,
wherein the combination of the first urolithin and the second urolithin is a combination of urolithin C and urolithin A, respectively.

31 . The production method according to claim 30 ,
wherein the urolithin C is obtained by allowing, in a solution containing a raw material of urolithin C, a microorganism having an ability to produce urolithin C from the raw material of urolithin C to produce urolithin C from the raw material of urolithin C.

32 . The production method according to claim 30 , further comprising the following Step (b1):
Step (b1): allowing, in a solution containing a raw material of urolithin C, a microorganism having an ability to produce urolithin C from the raw material of urolithin C to produce urolithin C from the raw material of urolithin C; wherein the Step (a) and the Step (b1) are carried out in the same system.

33 . The production method according to claim 31 ,
wherein the microorganism having an ability to produce urolithin C from the raw material of urolithin C is a microorganism belonging to the genus Gordonibacter.

34 . The production method according to claim 33 ,
wherein the microorganism belonging to the genus Gordonibacter is a microorganism belonging to Gordonibacter pamelaeae and/or a microorganism belonging to Gordonibacter urolithinfaciens.

35 . The production method according to claim 34 ,
wherein the microorganism belonging to Gordonibacter pamelaeae is the Gordonibacter pamelaeae DSM 19378 strain.

36 . The production method according to claim 34 ,
wherein the microorganism belonging to Gordonibacter urolithinfaciens is the Gordonibacter urolithinfaciens DSM 27213 strain.

37 . The production method according to claim 31 ,
wherein the raw material of urolithin C is ellagic acid and/or ellagitannin.

38 . The production method according to claim 23 ,
wherein the Step (a) is carried out in an environment with a gas phase containing hydrogen.

39 . The production method according to claim 38 ,
wherein the ratio of the hydrogen in the gas phase is not less than 0.5% and not more than 20%.

40 . The production method according to claim 30 ,
wherein, in the Step (a), the solution containing urolithin C further contains at least one selected from the group consisting of inclusion compounds of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.

41 . The production method according to claim 32 ,
wherein, in the Step (b1), the solution containing the raw material of urolithin C further contains at least one selected from the group consisting of inclusion compounds of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.

42 . A method for producing a food or drink containing urolithin A, comprising the following Steps (a1) and (c):
Step (a1): allowing, in a solution containing urolithin C, a microorganism having an ability to produce urolithin A from urolithin C to produce urolithin A from urolithin C; and Step (c): mixing the urolithin A produced in the Step (a1) with a raw material of the food or drink to prepare the food or drink.

Assignees

• Daicel Corp

Inventors

• Kudoh Masatake
• Nakajima Takanori
• Yamamoto Hiroaki

Classifications

• C12P17/06 Primary

  containing a six-membered hetero ring, e.g. fluorescein · CPC title

• A23L33/105

  Plant extracts, their artificial duplicates or their derivatives · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• C12R1/145

  Chemistry & Metallurgy · mapped topic

• C12R2001/145

  Clostridium · CPC title