Novel polypeptide and method of producing imp using the same

Patent metadata

Field	Value
Publication number	US-2019284595-A1
Application number	US-201916425897-A
Country	US
Kind code	A1
Filing date	May 29, 2019
Priority date	Dec 15, 2017
Publication date	Sep 19, 2019
Grant date	—

Abstract

The present disclosure relates to a novel polypeptide having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the same, a method for preparing 5′-inosine monophosphate using the same, and a method for increasing export of 5′-inosine monophosphate.

First claim

1 . A protein variant exporting 5′-inosine monophosphate, wherein, in the amino acid sequence of SEQ ID NO: 2, (i) the 2 nd amino acid, (ii) the 64 th amino acid, or (iii) the 2 nd amino acid and the 64 th amino acid are each substituted with another amino acid.

2 . (canceled)

3 . The protein variant according to claim 1 , wherein the 2 nd amino acid is substituted with an amino acid selected from the group consisting of isoleucine, phenylalanine, methionine, glutamic acid, histidine, and asparagine, (ii) the 64 th amino acid is substituted with an amino acid selected from the group consisting of aspartate, glutamic acid, asparagine, cysteine, isoleucine, and phenylalanine; or (iii) the 2 nd amino acid and the 64 th amino acid are each substituted with an amino acid selected from the group consisting of methionine, glutamic acid, histidine, asparagine, aspartate, cysteine, isoleucine, and phenylalanine.

4 . (canceled)

5 . A polynucleotide encoding the protein variant of claim 1 .

6 . A vector comprising the polynucleotide of claim 5 .

7 . A microorganism of the genus Corynebacterium producing 5′-inosine monophosphate, which comprises the protein variant of claim 1 ; or a vector comprising the polynucleotide encoding the protein variant of claim 1 .

8 . The microorganism of the genus Corynebacterium according to claim 7 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.

9 . (canceled)

10 . A method for preparing 5′-inosine monophosphate, comprising culturing the microorganism of the genus Corynebacterium of claim 7 in a medium; and recovering 5′-inosine monophosphate from the microorganism or medium.

11 . (canceled)

12 . The method according to claim 10 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.

13 . - 15 . (canceled)

16 . The microorganism of the genus Corynebacterium according to claim 7 , further comprising a protein variant, in which the 164 th amino acid of SEQ ID NO: 1 is substituted with another amino acid.

17 . The microorganism of the genus Corynebacterium according to claim 16 , wherein the 164 th amino acid of SEQ ID NO: 1 is substituted with an amino acid selected from the group consisting of lysine, arginine, asparagine, glycine, threonine, and proline.

18 . The microorganism of the genus Corynebacterium according to claim 16 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.

19 . A method for preparing 5′-inosine monophosphate, comprising culturing the microorganism of the genus Corynebacterium of claim 16 in a medium; and
recovering 5′-inosine monophosphate from the microorganism or medium.

20 . The method according to claim 19 , wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.

Assignees

• Cj Cheiljedang Corp

Inventors

• Kwon Jung Gun
• Baek Min Ji
• Lee Ji Hye
• Kwon Nara
• Kim Ju Jeong
• Rho Jin Ah
• Cho Jin Man

Classifications

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title

• C12P19/32 Primary

  having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide · CPC title

• C07K14/34

  from Corynebacterium (G) · CPC title

• C12R1/15

  Chemistry & Metallurgy · mapped topic

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title