Dispenser system for mass spectrometric sample preparations

Patent metadata

Field	Value
Publication number	US-2018374692-A1
Application number	US-201816121053-A
Country	US
Kind code	A1
Filing date	Sep 4, 2018
Priority date	Sep 13, 2013
Publication date	Dec 27, 2018
Grant date	—

Abstract

The invention relates to the preparation of samples on mass spectrometric sample supports with dispensing of liquids, and particularly to devices and methods to clean the dispenser. During dispensing of hundreds of samples, solved material may crystallize over time as deposit at the capillary tip of the dispenser, which impedes the vertical detachment of the drop in the medium and long run. Therefore, frequently cleaning the capillary tip is essential for a robust operation of the preparation device. The invention proposes to automatically clean the dispenser tip by creating a drop of washing fluid, such as pure solvent, fully enclosing the dispenser tip. The washing fluid is fed through a channel outside the central dispenser capillary to a location slightly above the capillary tip. The size of the hanging drop of washing fluid is photometrically regulated and monitored to prevent it from falling off prematurely.

First claim

1 . A method for cleaning a tip of a dispensing capillary, wherein a washing fluid is fed through at least one outer channel to a location slightly set back from the tip of the central dispensing capillary to form a drop fully enclosing the tip, wherein the size of the drop is monitored and regulated photometrically by a device having a light source and a light detector to prevent the drop of washing fluid from falling off prematurely.

2 . The method according to claim 1 , wherein the drop of washing fluid is formed at a horizontal plane being substantially wettable by the washing fluid so that the drop can fully enclose the tip even if a tip material is substantially unwettable by the washing fluid.

3 . The method according to claim 1 , wherein the drop of washing fluid is kept in motion by at least once drawing it partly into the dispensing capillary and pressing it out again with the aid of a dispensing micropump used to dispense liquids through the dispensing capillary.

4 . The method according to claim 1 , wherein an amount of light received by the light detector in a phase where no drop is pendant from an end of the at least one outer channel is used to detect deposits at the tip of the dispensing capillary.

5 . The method according to claim 1 , wherein some washing fluid is drawn into the dispensing capillary to protect the tip from being contaminated with crystallizing material during standby times.

6 . The method according to claim 1 , wherein, in certain phases of operation, a first micropump presses a drop of dispensing liquid out of the tip of the dispensing capillary and a detachment device detaches the drop of dispensing liquid from the tip of the dispensing capillary, and wherein, in alternate phases of operation, a second micropump presses the washing fluid through the at least one outer channel outside of the dispensing capillary to form the drop of washing fluid hanging from the end of the at least one outer channel and fully enclosing the dispensing capillary tip.

7 . The method according to claim 6 , wherein the detachment device for dispensing a drop from the tip of the dispensing capillary comprises one of a device to generate a pressure surge of a gas, a device for jerky detachment, a piezo device, and a vapor-bubble generator.

8 . The method according to claim 6 , wherein at least one of the first micropump and the second micropump is a peristaltic micropump or a piston pump.

9 . The method according to claim 1 , wherein the at least one outer channel ends in a flat horizontal plane a material of which is configured such that it has substantial adhesive properties for being wettable by the washing fluid, in order to enable the drop of washing fluid to fully enclose the dispenser tip even if a material of the dispenser tip is substantially unwettable by the washing fluid.

10 . The method according to claim 8 , wherein the material of the flat horizontal plane comprises metal.

11 . The method according to claim 1 , wherein the material of the dispensing capillary comprises polytetrafluoroethylene (PTFE).

12 . The method according to claim 1 , wherein the tip of the dispensing capillary projects into the light beam between light source and light detector to photometrically detect deposits when no drop is pendant from the capillary tip.

13 . The method according to claim 1 , wherein the light source and the light detector are located diametrically across a dispensing capillary axis.

14 . The method according to claim 1 , wherein the light source is one of a light-emitting diode (LED), a laser and a laser diode and comprises lenses or apertures as light-beam forming elements.

15 . The method according to claim 1 , wherein the light detector is one of a photoelectric cell and a charge-coupled device (CCD).

16 . The method according to claim 1 , wherein at least one of the first micropump and second micropump perform an oscillatory backward and forward pumping of liquid.

17 . The method according to claim 1 , wherein the at least one outer channel comprises one of an extra channel for supplying washing fluid and a gas channel for exerting gas pulses on drops hanging from, or at, the tip of the dispensing capillary.

18 . The method according to claim 1 , wherein the at least one outer channel for supplying washing fluid comprises two or more channels being arranged in a rotationally symmetric manner around the dispensing capillary.

19 . The method according to claim 1 , wherein a volume of the drop of washing fluid amounts to between about four and eight microliters.

Assignees

• Bruker Daltonik Gmbh

Inventors

• Schürenberg Martin
• Vossgröne Alexander

Classifications

• H01J49/02

  Details · CPC title

• B01L3/0241

  Drop counters; Drop formers · CPC title

• H01J49/0431 Primary

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title

• H01J49/0418

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title

• G01N35/1004

  Cleaning sample transfer devices · CPC title