Method for imaging biologic fluid samples using a predetermined distribution

Patent metadata

Field	Value
Publication number	US-2018348196-A1
Application number	US-201816102036-A
Country	US
Kind code	A1
Filing date	Aug 13, 2018
Priority date	Dec 6, 2012
Publication date	Dec 6, 2018
Grant date	—

Abstract

A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.

First claim

What is claimed is:

1 . A method for imaging a biologic fluid sample having one or more constituents, comprising:
providing a predetermined repeatable non-uniform spatial distribution representative of at least one constituent within the biologic fluid sample quiescently residing within an analysis chamber, wherein the distribution indicates a presence or absence of the at least one constituent residing in select sub-regions of the analysis chamber; and imaging the select sub-regions based on the presence or absence of the at least one constituent in the select sub-regions as indicated by the distribution.

2 . The method of claim 1 , wherein the imaging step further comprises selecting at least one imaging method for imaging the select sub-regions based on the presence or absence of the at least one constituent in the select sub-regions as indicated by the distribution.

3 . The method of claim 2 , wherein the imaging step further comprises creating image data representative of the biologic fluid sample in the select sub-regions, using the at least one imaging method.

4 . The method of claim 1 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

5 . The method of claim 4 , wherein the predetermined repeatable non-uniform spatial distribution of at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber indicates a plurality of sub-regions where white blood cells are likely present.

6 . The method of claim 4 , wherein the predetermined repeatable non-uniform spatial distribution of at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber indicates a plurality of sub-regions where red blood cells are likely present.

7 . The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber in the select one or more sub-regions is based on empirical data.

8 . The method of claim 1 , wherein the chamber is a microscope slide.

9 . An apparatus for imaging a biologic fluid sample, the apparatus comprising:
at least one sample illuminator; at least one image dissector; and at least one processor in communication with the at least one sample illuminator and the at least one image dissector, the processor in communication with stored instructions, which instructions include a predetermined repeatable non-uniform spatial distribution representative of at least one constituent within the biologic fluid sample quiescently disposed within an analysis chamber, wherein the spatial distribution indicates a presence or an absence of the at least one constituent residing in particular sub-regions of the chamber, and when executed the instructions cause the processor to image the select sub-regions based on the presence or absence of the at least one constituent in the select sub-regions as indicated by the distribution.

10 . The apparatus of claim 9 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

11 . The apparatus of claim 10 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently disposed within the chamber indicates a plurality of sub-regions where white blood cells are likely present.

12 . The apparatus of claim 10 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently disposed within the chamber indicates a plurality of sub-regions where red blood cells are likely present.

13 . The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber in the select one or more sub-regions is based on empirical data.

14 . The apparatus of claim 9 , wherein the chamber is a microscope slide.

Assignees

• Abbott Point Of Care Inc

Inventors

• Xie Min
• Wardlaw Stephen C

Classifications

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N15/0227

  using imaging; using holography · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G06K9/00134

  Physics · mapped topic