Method for imaging biologic fluid samples using a predetermined distribution

Patent metadata

Field	Value
Publication number	US-9885701-B2
Application number	US-201715398299-A
Country	US
Kind code	B2
Filing date	Jan 4, 2017
Priority date	Dec 6, 2012
Publication date	Feb 6, 2018
Grant date	Feb 6, 2018

Abstract

A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.

First claim

What is claimed is:

1. A method for imaging a biologic fluid sample having one or more constituents, which sample is quiescently disposed within an analysis chamber, comprising:
providing a spatial mapping of the analysis chamber, which spatial mapping defines a plurality of sub-regions of the analysis chamber;
providing a predetermined repeatable non-uniform spatial distribution of at least one of the one or more constituents within the sample relative to the chamber, wherein the distribution indicates a probability that the at least one of the one or more constituents within the sample resides in particular sub-regions of the analysis chamber;
selecting at least one imaging method for imaging one or more of the analysis chamber sub-regions based on the probability as indicated by the distribution; and
creating image data representative of the biologic fluid sample in select chamber sub-regions, using the at least one imaging method selected for the respective one or more of the select chamber sub-regions.

2. The method of claim 1 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

3. The method of claim 1 , wherein the chamber is a microscope slide.

4. The method of claim 1 , wherein the step of creating image data representative of the biologic fluid sample in select chamber sub-regions includes creating image data representative of the biologic fluid sample in all chamber sub-regions.

5. The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where a number of white blood cells reside within the chamber.

6. The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where a number of red blood cells reside within the chamber.

7. The method of claim 1 , further comprising analyzing the biologic fluid sample, using the image data representative of the biologic fluid sample in the select chamber sub-regions.

8. The method of claim 1 , wherein the probability that the at least one of the one or more constituents within the sample resides in particular sub-regions of the analysis chamber is based on empirical data.

9. An apparatus for imaging a biologic fluid sample, comprising:
a chamber operable to quiescently hold the biologic fluid sample, which chamber is spatially mapped to divide the chamber into a plurality of sub-regions;
a sample illuminator;
at least one image dissector operable to produce image signals representative of the sample residing within the chamber; and
a processor in communication with the sample illuminator, the at least one image dissector, and a non-transitory memory storing instructions, which instructions include a predetermined repeatable non-uniform spatial distribution of one or more constituents within the fluid sample quiescently disposed within the chamber, wherein the spatial distribution indicates a probability that the at least one of the one or more constituents within the sample resides in particular sub-regions of the analysis chamber, and when executed the instructions cause the processor to:
select at least one imaging method for imaging one or more of the analysis chamber sub-region based on the probability as indicated by the distribution;
control the sample illuminator and the image dissector to create image data representative of the biologic fluid sample in select chamber sub-regions, using the at least one imaging method selected for the respective one or more of the select chamber sub-regions.

10. The apparatus of claim 9 , wherein the chamber is a microscope slide.

11. The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where the number of white blood cells reside within the chamber.

12. The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where a number of red blood cells reside within the chamber.

13. The apparatus of claim 9 , wherein the number of at least one of the constituents within the sample in each sub-region of the chamber is based on empirical data.

14. An apparatus for imaging a biologic fluid sample, comprising:
a chamber operable to quiescently hold the biologic fluid sample;
a sample illuminator;
at least one image dissector operable to produce image signals representative of the sample residing within the chamber; and
a processor in communication with the sample illuminator, the at least one image dissector, and a non-transitory memory storing instructions, which instructions include a predetermined repeatable non-uniform spatial distribution of one or more constituents within the fluid sample quiescently disposed within the chamber, wherein the spatial distribution indicates a probability that the at least one of the one or more constituents within the sample resides in particular sub-regions of the analysis chamber, and when executed the instructions cause the processor to:
apply a spatial map to the analysis chamber that defines the chamber sub-regions;
select at least one imaging method for imaging one or more of the analysis chamber sub-regions based on the probability as indicated by the distribution;
control the sample illuminator and the image dissector to create image data representative of the biologic fluid sample in select chamber sub-regions, using the at least one imaging method selected for the respective one or more of the select chamber sub-regions.

15. The apparatus of claim 14 , wherein the chamber is a microscope slide.

16. The apparatus of claim 14 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where the number of white blood cells reside within the chamber.

17. The apparatus of claim 14 , wherein the predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample disposed within the chamber indicates a plurality of sub-regions where a number of red blood cells reside within the chamber.

18. The apparatus of claim 14 , wherein the number of at least one of the constituents within the sample in each sub-region of the chamber is based on empirical data.

Assignees

• Abbott Point Of Care Inc

Inventors

• Xie Min
• Wardlaw Stephen C

Classifications

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N15/0227

  using imaging; using holography · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N15/1463

  Physics · mapped topic