Method for producing heavy chain diol

Patent metadata

Field	Value
Publication number	US-2018340195-A1
Application number	US-201615771799-A
Country	US
Kind code	A1
Filing date	Oct 27, 2016
Priority date	Oct 27, 2015
Publication date	Nov 29, 2018
Grant date	—

Abstract

The present invention relates to a method for producing medium chain diol and, more particularly to recombinant microorganisms in which fatty alcohol dehydrogenase and/or fatty alcohol oxidase genes on a ω-oxidative metabolism pathway are deleted, the fatty aldehyde dehydrogenase genes are optionally deleted, and β-oxidative metabolism pathway-related genes are deleted, and to a method for producing medium chain diol from fatty acid-derived alcohol or alkane by culturing the recombinant microorganisms. The recombinant microorganisms of the present invention can produce a high yield of medium chain diol by preventing further oxidation and β-oxidative metabolism of fatty alcohols.

First claim

1 . A recombinant microorganism from which one or more genes selected from the group consisting of fatty alcohol dehydrogenase and fatty alcohol oxidase genes in an ω-oxidative metabolism pathway are deleted, a fatty aldehyde dehydrogenase gene is optionally deleted, and β-oxidative metabolism pathway-related genes are also deleted.

2 . The recombinant microorganism of claim 1 , wherein the fatty alcohol dehydrogenase gene, the fatty alcohol oxidase gene, the fatty aldehyde dehydrogenase gene, and the β-oxidative metabolism pathway-related genes are deleted from all homologous genes present in the microorganism.

3 . The recombinant microorganism of claim 1 , wherein the fatty alcohol dehydrogenase gene, the fatty alcohol oxidase gene, the fatty aldehyde dehydrogenase gene, and the β-oxidative metabolism pathway-related genes are deleted from some of the homologous genes present in the corresponding microorganism.

4 . The recombinant microorganism of claim 1 , wherein the fatty alcohol dehydrogenase gene is selected from the group consisting of ADH1, ADH2, ADH3, ADH4, ADH5, ADH6, ADH7, ADH8, and FADH genes.

5 . The recombinant microorganism of claim 4 , wherein each of the ADH1, ADH2, ADH3, ADH4, ADH5, ADH6, ADH7, ADH8 and FADH genes comprises base sequences set forth in SEQ ID NOs: 1 to 9, respectively.

6 . The recombinant microorganism of claim 1 , wherein the fatty alcohol oxidase gene is an FAO gene.

7 . The recombinant microorganism of claim 6 , wherein the FAO gene comprises a base sequence set forth in SEQ ID NO: 10.

8 . The recombinant microorganism of claim 1 , wherein the fatty aldehyde dehydrogenase gene is selected from the group consisting of FALDH1, FALDH2, FALDH3, and FALDH4 genes.

9 . The recombinant microorganism of claim 8 , wherein each of the FALDH1, FALDH2, FALDH3, and FALDH4 genes comprises base sequences set forth in SEQ ID NOs: 11 to 14, respectively.

10 . The recombinant microorganism of claim 1 , wherein the β-oxidative metabolism pathway-related gene is an acyl-CoA oxidase gene.

11 . The recombinant microorganism of claim 10 , wherein the acyl-CoA oxidase gene is selected from the group consisting of ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes.

12 . The recombinant microorganism of claim 11 , wherein each of the ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes comprises base sequences set forth in SEQ ID NOs: 15 to 20, respectively.

13 . The recombinant microorganism of claim 1 , wherein the microorganism is a yeast or Escherichia coli.

14 . The recombinant microorganism of claim 13 , wherein the yeast is selected from the group of the yeast consisting of Yarrowia sp., Saccharomyces sp., Pichia sp., and Candida sp.

15 . The recombinant microorganism of claim 14 , wherein the yeast of Yarrowia sp. is Yarrowia lipolytica.

16 . A method for producing a medium chain diol, comprising:
(1) preparing the recombinant microorganism according to claim 1 ; and (2) treating the recombinant microorganism with a substrate to culture the recombinant microorganism.

17 . The method of claim 16 , wherein the substrate is selected from the group consisting of a fatty acid-derived alcohol and alkane.

18 . The method of claim 17 , wherein the fatty acid-derived alcohol is an alcohol having 5 to 30 carbon atoms.

19 . The method of claim 17 , wherein the alkane is an alkane having 5 to 30 carbon atoms.

20 . The method of claim 18 , wherein the medium chain diol is a diol compound having 5 to 30 carbon atoms.

Assignees

• Korea Res Inst Bioscience & Biotechnology

Inventors

• Ahn Jung Oh
• Lee Hong Weon
• Jang Min Jeong
• Kim Chun Sug
• Park Gyu Yeon

Classifications

• C12N15/81

  for yeasts · CPC title

• C12N9/001

  acting on the CH-CH group of donors (1.3) · CPC title

• C12N9/0006 Primary

  acting on CH-OH groups as donors (1.1) · CPC title

• C12Y103/03006

  Acyl-CoA oxidase (1.3.3.6) · CPC title

• C12P7/18 Primary

  polyhydric · CPC title