Ultrasound-assisted electrochemical distinction of normal and cancerous cells

Patent metadata

Field	Value
Publication number	US-2018252677-A1
Application number	US-201815960542-A
Country	US
Kind code	A1
Filing date	Apr 23, 2018
Priority date	Apr 24, 2017
Publication date	Sep 6, 2018
Grant date	—

Abstract

A method for cancer diagnosis is disclosed. The method includes forming a plurality of cultured cells on an electrochemical biosensor placing the electrochemical biosensor in a medium solution comprising a cell culture solution of a plurality of biological cells, measuring a first electrochemical response from the electrochemical biosensor with the plurality of cultured cells, forming a plurality of stimulated cells on the electrochemical biosensor by ultrasonically stimulating of the plurality of cultured cells, measuring a second electrochemical response from the electrochemical biosensor with the plurality of stimulated cells, and detecting presence of cancer cells responsive to a difference between the first electrochemical response and the second electrochemical response being less than a threshold. Where, the first electrochemical response includes an electrochemical response of the plurality of cultured cells and the second electrochemical response includes an electrochemical response of the plurality of stimulated cells.

First claim

What is claimed is:

1 - A method for cancer diagnosis, comprising:
preparing an electrochemical-ultrasonic system, comprising:
fabricating an electrochemical biosensor;
culturing a plurality of biological cells on the electrochemical biosensor by placing the electrochemical biosensor in a medium solution, the medium solution comprising a cell culture solution of the plurality of biological cells;
connecting the electrochemical biosensor with the plurality of cultured cells to an electrochemical stimulator-analyzer system, the electrochemical stimulator-analyzer system configured to measure electrochemical responses; and
exposing the electrochemical biosensor with the plurality of cultured cells to an ultrasonic system;
generating a plurality of microbubbles on the electrochemical biosensor with the plurality of cultured cells by applying an instantaneous electrical potential in the medium solution; measuring a first electrochemical response from the electrochemical biosensor with the plurality of cultured cells using the electrochemical stimulator-analyzer system, the first electrochemical response comprising an electrochemical response of the plurality of cultured cells; forming a plurality of stimulated cells on the electrochemical biosensor by applying ultrasonic waves to the plurality of cultured cells and the plurality of microbubbles using the ultrasonic system; measuring a second electrochemical response from the electrochemical biosensor with the plurality of stimulated cells using the electrochemical stimulator-analyzer system, the second electrochemical response comprising an electrochemical response of the of the plurality of stimulated cells; and detecting presence of cancer cells responsive to a difference between the first electrochemical response and the second electrochemical response being less than a threshold.

2 - A method for cancer diagnosis, comprising:
forming a plurality of cultured cells on an electrochemical biosensor by placing the electrochemical biosensor in a medium solution comprising a cell culture solution of a plurality of biological cells; measuring a first electrochemical response from the electrochemical biosensor with the plurality of cultured cells, the first electrochemical response comprising an electrochemical response of the plurality of cultured cells; forming a plurality of stimulated cells on the electrochemical biosensor by ultrasonically stimulating of the plurality of cultured cells; measuring a second electrochemical response from the electrochemical biosensor with the plurality of stimulated cells, the second electrochemical response comprising a electrochemical response of the plurality of stimulated cells; and detecting presence of cancer cells responsive to a difference between the first electrochemical response and the second electrochemical response being less than a threshold.

3 - The method of claim 2 , wherein each of the first electrochemical response and the second electrochemical response comprises an electrical current, and
wherein the difference between the first electrochemical response and the second electrochemical response comprises

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being defined by:

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where I US off comprises the first electrochemical response and I US on comprises the second electrochemical response.

4 - The method of claim 2 , wherein the threshold comprises a value of 0.15.

5 - The method of claim 2 , wherein the threshold comprises a value of 0.05 for the ultrasonic waves with an intensity of less than 1 W/cm 2 and a duration of less than 5 seconds, and
wherein the threshold comprises a value of 0.15 for the ultrasonic waves with an intensity of more than 1 W/cm 2 and a duration of less than 5 seconds.

6 - The method of claim 2 , wherein each of the measuring the first electrochemical response from the electrochemical biosensor with the plurality of cultured cells and measuring the second electrochemical response from the electrochemical biosensor with the plurality of stimulated cells comprise measuring an electrochemical response via a cyclic voltammetry (CV) technique using an electrochemical stimulator-analyzer system.

7 - The method of claim 6 , wherein the electrochemical stimulator-analyzer system comprises a potentiostat.

8 - The method of claim 2 , wherein forming the plurality of cultured cells on the electrochemical biosensor comprises:
placing the electrochemical biosensor at the bottom of a chamber; and filling the chamber with the medium solution, the medium solution comprising a cell culture solution of the plurality of biological cells.

9 - The method of claim 2 , further comprises generating a plurality of microbubbles on the electrochemical biosensor with the plurality of cultured cells.

10 - The method of claim 9 , wherein generating the plurality of microbubbles on the electrochemical biosensor with the plurality of cultured cells comprises electrolysis of the medium solution by applying an instantaneous electrical potential to the electrochemical biosensor with the plurality of cultured cells via a cyclic voltammetry technique using an electrochemical stimulator-analyzer system.

11 - The method of claim 10 , wherein generating the plurality of microbubbles on the electrochemical biosensor with the plurality of cultured cells comprises applying a DC signal with a voltage between −2 V and −0.5 V for a time duration less than 1 seconds on the biosensor with the plurality of cultured cells using a potentiostat device.

12 - The method of claim 9 , wherein forming the plurality of stimulated cells on the electrochemical biosensor by ultrasonically stimulating of the

Assignees

• Abdolahad Mohammad
• Khayamian Mohammad Ali
• Ansaryan Saeid
• Baniassadi Majid

Inventors

• Abdolahad Mohammad
• Khayamian Mohammad Ali
• Ansaryan Saeid
• Baniassadi Majid

Classifications

• G01N27/48 Primary

  using polarography, i.e. measuring changes in current under a slowly-varying voltage · CPC title

• G01N33/4833

  of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735) · CPC title

• G01N27/327 Primary

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• C23C14/205

  by cathodic sputtering · CPC title

• C23C14/022

  by means of bombardment with energetic particles or radiation · CPC title