System for hypothermic transport of samples

Patent metadata

Field	Value
Publication number	US-2018132478-A1
Application number	US-201815870209-A
Country	US
Kind code	A1
Filing date	Jan 12, 2018
Priority date	Mar 15, 2011
Publication date	May 17, 2018
Grant date	—

Abstract

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

First claim

1 . A system for transporting a biological sample, comprising:
a first transport container comprising:
a pumping chamber having a semi-permeable membrane configured to push against a fluid and cause the fluid to circulate inside the first transport container;
a sample storage chamber, configured to receive a biological sample, and configured to interface with and be in fluidic communication with the pumping chamber;
a fill port providing a fluid path between the exterior of the first transport container and the sample storage chamber; and
a vent port providing a fluid path between the exterior of the first transport container and the pumping chamber; and
a second transport container comprising an insulated cavity for receiving the first transport container and having recesses for receiving cooling media.

2 . The system of claim 1 , wherein the semi-permeable membrane is disposed at an inclined angle with respect to horizontal when the first transport container is placed on a horizontal surface.

3 . The system of claim 2 , wherein the semi-permeable membrane is inclined at an angle between approximately 1°-30° with respect to horizontal.

4 . The system of claim 1 , further comprising a valve in communication with the sample storage container and the pumping chamber.

5 . The system of claim 4 , wherein the valve is a ball check valve configured to allow fluid flow from the sample storage chamber to pumping chamber.

6 . The system of claim 1 , further comprising a source of oxygen-containing gas.

7 . The system of claim 6 , wherein the source of oxygen-containing gas is in fluid communication with a first side of the semi-permeable membrane and configured to provide a force against the semi-permeable membrane, thereby causing a second side of the semi-permeable membrane to push against the fluid and cause the fluid to circulate inside the first transport container.

8 . The system of claim 6 , wherein the source of an oxygen-containing gas is a compressed gas cylinder.

9 . The system of claim 1 , further comprising a third transport container configured to enclose the first transport container and fit within the second transport container.

10 . The system of claim 9 , wherein the third transport container is configured to be sterilized.

11 . The system of claim 1 , wherein the biological sample comprises tissues or organs.

12 . The system of claim 1 , wherein the biological sample is a container holding body fluids.

13 . The system of claim 1 , further comprising an adapter configured to couple the biological sample to the pumping chamber.

14 . The system of claim 1 , further comprising a temperature sensor, a pressure sensor, or an oxygen sensor.

15 . The system of claim 14 , further comprising a display.

16 . The system of claim 15 , wherein the display communicates with the temperature sensor, the pressure sensor, or the oxygen sensor wirelessly.

17 . The system of claim 1 , wherein the cooling media comprises eutectic cooling packs.

18 . The use of a system of any of claims 1 to 17 with a preservation solution comprising glucose, histidine, lactobionate, mannitol, raffinose, or sucrose.

19 . The use of claim 18 , wherein the preservation solution is selected from the group consisting of Collins, EuroCollins, phosphate buffered sucrose (PBS), University of Wisconsin (UW), histidine-tryptophan-ketoglutarate (HTK), hypertonic citrate, hydroxyethyl starch, and Celsior.

Assignees

• Paragonix Tech Inc

Inventors

• Anderson Lisa Maria
• Judson Jared Alden

Classifications

• C12M45/22

  Means for packing or storing viable microorganisms (casings for storing cell samples A61B10/0096, preservation of living parts of the human or animal body A01N1/10) · CPC title

• A01N1/0247

  Human Necessities · mapped topic

• A01N1/0226 Primary

  Human Necessities · mapped topic

• A01N1/162

  Temperature processes, e.g. following predefined temperature changes over time · CPC title

• A01N1/143 Primary

  for organ perfusion · CPC title