Reaction Vessel for Sample Preparation

Patent metadata

Field	Value
Publication number	US-2018067021-A1
Application number	US-201715703353-A
Country	US
Kind code	A1
Filing date	Sep 13, 2017
Priority date	Dec 19, 2012
Publication date	Mar 8, 2018
Grant date	—

Abstract

The present invention relates to sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, the container comprising a reaction chamber and a chromatography medium; wherein said reaction chamber is for holding said cellular material, viruses and/or sub-components of said cellular material and/or viruses and is configured such that at least one of the following reactions can be performed therein: lysis, e.g. by sonication and/or boiling; chromatographic purification; reduction; alkylation; and enzymatic reactions such as proteolysis; wherein said chromatography medium is configured to purify and/or enrich said bio-organic compounds; wherein (a) said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds; or (b) said sample preparation container further comprises a receiving chamber for receiving said bio-organic compounds, said receiving chamber being adjacent to said chromatography medium such that said chromatography medium separates said reaction chamber from said receiving chamber, and the outer face of said receiving chamber is closed and configured to be opened for obtaining purified and/or enriched bio-organic compounds.

First claim

1 . Sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, the container comprising a reaction chamber and a chromatography medium;
wherein said reaction chamber is for holding said cellular material, viruses and/or sub-components of said cellular material and/or viruses and is configured such that at least one of the following reactions can be performed therein: lysis; chromatographic purification; reduction; alkylation; and an enzymatic reaction; wherein said chromatography medium is configured to purify and/or enrich said bio-organic compounds; wherein (a) said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds; or (b) said sample preparation container further comprises a receiving chamber for receiving said bio-organic compounds, said receiving chamber being adjacent to said chromatography medium such that said chromatography medium separates said reaction chamber from said receiving chamber, and the outer face of said receiving chamber is closed and configured to be opened for obtaining purified and/or enriched bio-organic compounds.

2 . The sample preparation container of claim 1 , wherein said bio-organic compounds comprise at least one of the following: proteins; peptides; polypeptides; nucleic acids, e.g. deoxyribonucleic acids and ribonucleic acids; lipids including fatty acids; and metabolites.

3 . The sample preparation container of claim 1 further comprising a lid, said lid being adjacent to said reaction chamber and configured to allow feeding of a sample, e.g. by penetration with a needle, and preferably configured to self-reseal after feeding.

4 . The sample preparation container of claim 1 , wherein the surface of said chromatography medium is configured to act as a filtration surface and/or comprises a further filtration layer and/or reactive layer, said surface or layer facing the interior of said reaction chamber.

5 . The sample preparation container of claim 1 comprising a receiving chamber for receiving said bio-organic compounds, said receiving chamber being adjacent to said chromatography medium such that said chromatography medium separates said reaction chamber from said receiving chamber, and the outer face of said receiving chamber is closed and configured to be opened for obtaining purified and/or enriched bio-organic compounds, and further comprising a seal wherein said seal is adjacent to said receiving chamber and configured for obtaining said purified and/or enriched bio-organic compounds.

6 . The sample preparation container of claim 1 , wherein said container comprises at least one of: polypropylene; polyethylene; a material configured to conduct heat, microwaves, shockwaves, sound waves and/or electricity; a material with low binding surface; a material which is transparent, opaque or selectively transmitting electromagnetic radiation, preferably light.

7 . The sample preparation container of claim 1 , wherein at least one inner wall of the sample preparation container has at least partially low binding and/or low retention characteristics, preferably by an at least partial coating with polytetrafluoroethylene.

8 . A receiving chamber configured to be coupled to the sample preparation container according to claim 1 , wherein said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds and that is configured to receive purified and/or enriched bio-organic compounds.

9 . The receiving chamber according to claim 8 comprising a coupling element, which is configured to be coupled to a corresponding coupling element of a sample preparation container wherein said chromatography medium is located at a wall of said reaction chamber, and said wall is closed or sealed and configured to be opened for obtaining purified and/or enriched bio-organic compounds.

10 .- 11 . (canceled)

12 . The sample preparation container of claim 1 , wherein said chromatography medium comprises
(a) at least one of the following materials: reversed-phase materials, cation-exchange materials, anion-exchange materials, mixed-mode ion-exchange materials, ion-complexing materials, an affinity-coupled matrix being antibody-coupled and/or lectin-coupled; and/or (b) two or more stacks of different chromatography media.

13 . The sample preparation container of claim 1 , wherein
(a) said bio-organic compounds comprise one or more proteins, peptides and/or polypeptides and said container is characterized by one, two, three, four, five, six, seven or all of the following (i) to (viii):
(i) said container comprises a detergent;
(ii) said container comprises a reducing agent;
(iii) said container comprises an alkylating agent;
(iv) the pH-value in said container is between 7 and 9;
(v) said container comprises a standard for mass-spectrometric analysis;
(vi) said container comprises a chaotropic agent;
(vii) said container comprises an analyte stabilizing chemical; and
(viii) said container comprises at least one enzyme selected from proteases; glycosidases; and kinases; and/or
(b) said bio-organic compounds comprise nucleic acids and said container is characterized by one, two or all of the following:
(i) said container comprises one or more nucleases;
(ii) said container comprises reagents for nucleic acid amplification; and
(iii) the pH-value in said container is between 7 and 9.

14 . The sample preparation container of claim 1 , said container further comprising
(a) at least one of the following chemicals: milling beads, detergents, chaotropic agents, alkylating agents, reducing agents, organic solvents, standards for mass-spectrometric analysis; and/or (b) at least one of the following enzymes: a protease, a nuclease, a decarboxylase.

15 . The sample preparation container of claim 1 , wherein
(a) said cellular material is one of: intact cells, non-clarified cell lysate, tissue, pathogens; and/or (b) said sub-components are sub-cellular structures, in particular organelles.

16 .- 19 . (canceled)

20 . A kit comprising
(a) a sample preparation container according to claim 1 ; and (b)(i) a protease; an alkylating agent; a reducing agent; a standard for mass-spectrometric analysis; a chaotropic agent, a detergent; and/or means for establishing a pH-value in said container of between 7 and 9; and/or
(ii) a nuclease; and/or reagents for nucleic acid amplification.

21 . The kit of claim 20 , further comprising
(a) at least one of the following: A bead-milling material, a detergent, a chaotropic agent, an alkylating agent, a reducing agent, an organic solvent, an antioxidant, a UV absorbant, a standard for mass-spectrometric analysis; (b) at least one of the following enzymes: a protease, a nuclease, a decarboxylase, a kinase, a glycosidase; and/or (c) a manual with instructions for performing the method of claim 10 .

22 . A system comprising
(a) a sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, the container comprising a reaction chamber and a chromatography medium; wherein said reaction chamber is for holding said cellular material, viruses and/or sub-compounds of said cel

Assignees

• Max Planck Gesellschaft

Inventors

• Kulak Nils A
• Mann Matthias
• Azimifar Seyed Babak
• Nagaraj Nagarjuna

Classifications

• B01D15/125

  Pre-filtration · CPC title

• B01D15/12

  relating to the preparation of the feed · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L2300/069

  Absorbents; Gels to retain a fluid · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title