Automated method of calibrating a chromatography system and analysis of a sample

Patent metadata

Field	Value
Publication number	US-2018024101-A1
Application number	US-201715716308-A
Country	US
Kind code	A1
Filing date	Sep 26, 2017
Priority date	Sep 18, 2014
Publication date	Jan 25, 2018
Grant date	—

Abstract

An automated method of calibrating a chromatography system and analyzing a sample is described. The method includes forming diluted standard solutions that are injected into a chromatography column. The detected peaks can be identified based on a first predetermined calibration ratio associated with the standard solution. Once the chromatography system is calibrated, samples can be chromatographically analyzed where the measured peaks are identified and quantified in an automated manner.

First claim

What is claimed is:

1 . A method of calibrating a chromatography system, the method comprising:
forming a first diluted standard solution by mixing a standard material and a diluent, the first diluted standard solution comprising:
a first analyte having a first diluted calibrant concentration, and
a second analyte having a second diluted calibrant concentration,
in which the first diluted standard solution is configured to have a first predetermined calibrant ratio;

injecting the first diluted standard solution into a chromatographic separator; separating the first diluted standard solution in the chromatographic separator; measuring, with a detector, a first peak and a second peak that have a first retention time and a second retention time, respectively; and automatically identifying
whether the first peak corresponds to the first analyte or the second analyte, and
whether the second peak corresponds to the first analyte or the second analyte, based on
either an area or a height of the first peak and the second peak, and
the first predetermined calibrant ratio;

forming a second diluted standard solution by mixing the standard material and the diluent, the second diluted standard solution comprising:
the first analyte having a third diluted calibrant concentration, and
the second analyte having a fourth diluted calibrant concentration;
injecting the second diluted standard solution into the chromatographic separator; separating the second diluted standard solution in the chromatographic separator; measuring, with the detector, a third peak and a fourth peak that have a third retention time and a fourth retention time, respectively; automatically identifying
whether the third peak corresponds to the first analyte or the second analyte, and
whether the fourth peak corresponds to the first analyte or the second analyte, based on
either an area or a height of the third peak and the fourth peak, and
the first predetermined calibrant ratio;

automatically calculating a first calibration equation for the first analyte based on
an area or a height of two of the automatically identified peaks that correspond to the first analyte, the two peaks selected from the group consisting of the first peak, the second peak, the third peak, and the fourth peak, and
the first and third diluted calibrant concentrations; and
automatically calculating a second calibration equation for the second analyte based on
an area or a height of two of the automatically identified peaks that correspond to the second analyte, the two peaks selected from the group consisting of the first peak, the second peak, the third peak, and the fourth peak, and
the second and fourth diluted calibrant concentrations.

2 . The method of claim 1 , in which the first calibration equation comprises a first calibration slope and a first y-intercept and the second calibration equation comprises a second calibration slope and a second y-intercept.

3 . The method of claim 1 further comprising:
assigning a first analyte time interval based on one of the first retention time and the second retention time that corresponds to the first analyte;
assigning a second analyte time interval based on one of the first retention time and the second retention time that corresponds to the second analyte;
injecting a first sample into the chromatographic separator, the first sample comprising at least a first analyte;
separating the first sample in the chromatographic separator;
measuring, with the detector, a fifth peak that has a fifth retention time;
automatically identifying that the fifth peak corresponds to the first analyte where the fifth retention time falls within the first analyte time interval; and
automatically calculating a first analyte concentration of the first sample based on an area or a height of the fifth peak and the first calibration equation.

4 . The method of claim 3 further comprising:
calculating a fifth diluted calibrant concentration for the first analyte that is less than the automatically calculated first analyte concentration when the automatically calculated first analyte concentration is less than a lower first analyte concentration of a first concentration range;
forming a third diluted standard solution by mixing the standard solution and the diluent, the third diluted standard solution comprising a first analyte having the fifth diluted calibrant concentration;
injecting the third diluted standard solution into the chromatographic separator;
separating the third diluted standard solution in the chromatographic separator;
measuring, with the detector, a sixth peak that has a sixth retention time;
automatically identifying that the sixth peak corresponds to the first analyte where the sixth retention time falls within the first analyte time interval; and
automatically calculating an adjusted first calibration equation for the first analyte based on
an area or a height of the sixth peak and at least one peak of the first, second, third, and fourth peaks that corresponds to the first analyte, and
the fifth diluted calibrant concentration and at least one of the first, second, third, and fourth diluted calibrant concentrations that corresponds to the first analyte.

5 . The method of claim 1 , in which the standard material includes a standard solution, the forming of the first diluted standard solution comprises:
pumping a first aliquot of the standard solution into a junction, the standard solution comprising:
the first analyte having the first calibrant concentration, and
the second analyte having the second calibrant concentration;
pumping a second aliquot of the diluent into the junction; and mixing the first aliquot and the second aliquot to form the first diluted standard solution; and the forming of the second diluted standard solution comprises: pumping a third aliquot of the standard solution into the junction; pumping a fourth aliquot of the diluent into the junction; and mixing the third aliquot and fourth aliquot to form the second diluted standard solution.

6 . The method of claim 5 further comprising:
before forming the first diluted standard solution and the second diluted standard solution, receiving a first concentration range for the first analyte, the first concentration range including a lower first analyte concentration and an upper first analyte concentration;
automatically calculating the first diluted calibrant concentration based on the upper first analyte concentration;
automatically setting
a first flow rate or a first pump duration time for the first aliquot and
a second flow rate or a second pump duration time for the second aliquot to form the automatically calculated first diluted calibrant concentration;

automatically calculating the third diluted calibrant concentration based on the lower first analyte concentration; and
automatically setting
a third flow rate or a third pump duration time for the third aliquot and
a fourth flow rate or a fourth pump duration time for the fourth aliquot to form the automatically calculated third diluted calibrant concentration.

7 . The method of claim 6 , in which the junction comprises a T-junction.

8 . The method of claim 7 , in which the mixing occurs in a mixing chamber downstream of the T-junction.

Assignees

• Dionex Corp

Inventors

• Srinivasan Kannan
• Pohl Christopher A

Classifications

• G01N30/8675

  Evaluation, i.e. decoding of the signal into analytical information (for analysis of specific compounds see also G01N30/88 and subgroups of G01N33/00; chemical libraries per se C40B) · CPC title

• G01N30/96

  using ion-exchange (G01N30/02, G01N30/90 take precedence) · CPC title

• G01N30/8668 Primary

  using retention times · CPC title

• G01N2030/047

  external · CPC title