Composition for preparing tagatose and method for preparing tagatose from fructose

Patent metadata

Field	Value
Publication number	US-2017306370-A1
Application number	US-201515521243-A
Country	US
Kind code	A1
Filing date	Oct 19, 2015
Priority date	Oct 22, 2014
Publication date	Oct 26, 2017
Grant date	—

Abstract

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

First claim

1 - 20 . (canceled)

21 . A method of producing tagatose, the method comprising:
contacting fructose with a protein or a microorganism expressing the protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 7 such that the fructose is enzymatically converted to tagatose.

22 . The method of producing tagatose according to claim 21 , wherein contacting is performed under at least one of the following conditions:
at a temperature between 50° C. and 90° C., at a pH between 6.0 and 10.0, and at a fructose concentration of 5% (w/v) to 60% (w/v).

23 . The method according to claim 22 , wherein the protein has amino acid sequence of SEQ ID NO: 7, wherein contacting is performed at a temperature between 50° C. and 80° C.

24 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 1, 3, 5, 6 or 7, wherein contacting is performed at a temperature between 60° C. and 80° C.

25 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 2 or 4, wherein contacting is performed at a temperature between 70° C. and 90° C.

26 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 1 or 6, wherein contacting is performed at a pH between 6.0 and 9.0.

27 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 4 or 7, wherein contacting is performed at a pH between 7.0 and 10.0.

28 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 1, 2, 3, or 5, wherein contacting is performed at a pH between 7.0 and 9.0.

29 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 4 or 7, wherein contacting is performed at a pH between 8.0 and 10.0.

30 . The method according to claim 22 , wherein the protein has an amino acid sequence of SEQ ID NO: 6, wherein contacting is performed at a pH between 6.0 and 8.0.

31 . The method of producing tagatose according to claim 21 , further comprising adding magnesium ions, zinc ions, nickel ions, cobalt ions, iron ions, manganese ions or a mixture of two or more of the foregoing.

32 . The method according to claim 21 , further comprising converting fructose into tagatose through epimerization at carbon-4 position of fructose.

33 . The method according to claim 21 , wherein in place of the protein to be used in the contacting step, a microorganism expressing protein having an amino acid sequence set forth in any one of SEQ ID NOs: 1 to 7 is used.

34 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO:1 is derived from Rhodothermus marinus.

35 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 2 is derived from Thermotoga petrophila.

36 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 3 is derived from Thermotoga lettingae.

37 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 4 is derived from Thermoanaerobacter mathranii.

38 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 5 is derived from Dictyoglomus turgidum.

39 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 6 is derived from Thermoanaerobacterium xylanolyticum.

40 . The method according to claim 21 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 7 is derived from Thermoanaerobacter siderophilus.

Assignees

• Cj Cheiljedang Corp

Inventors

• Kim Yang Hee
• Yang Sung Jae
• Lee Young Mi
• Park Il Hyang
• Kim Seong Bo
• Cho Hyun Kug
• Park Seung Won

Classifications

• C12N9/90 Primary

  Isomerases (5.) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C07K14/195

  from bacteria · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P19/24

  produced by the action of an isomerase, e.g. fructose · CPC title