Enzymatic synthesis of soluble glucan fiber

Patent metadata

Field	Value
Publication number	US-2017198322-A1
Application number	US-201515313263-A
Country	US
Kind code	A1
Filing date	May 22, 2015
Priority date	May 29, 2014
Publication date	Jul 13, 2017
Grant date	—

Abstract

An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

First claim

What is claimed is:

1 . A soluble α-glucan fiber composition comprising:
a. 10 to 20% α-(1,4) glycosidic linkages;
b. 60 to 88% α-(1,6) glycosidic linkages;
c. 0.1 to 15% α-(1,4,6) and α-(1,2,6) glycosidic linkages;
d. a weight average molecular weight of less than 50000 Daltons;
e. a viscosity of less than 0.25 Pascal second (Pa·s) at 12 wt % in water;
f. a digestibility of less than 12% as measured by the Association of Analytical Communities (AOAC) method 2009.01;
g. a solubility of at least 20% (w/w) in pH 7 water at 25° C.; and
h. a polydispersity index of less than 10.

2 . The soluble α-glucan fiber composition of claim 1 wherein the soluble α-glucan fiber composition is characterized by a number average molecular weight (Mn) between 1000 and 5000 g/mol.

3 . A carbohydrate composition comprising: 0.01 to 99 wt % (dry solids basis) of the soluble α-glucan fiber composition of claim 1 .

4 . The carbohydrate composition of claim 3 further comprising: a monosaccharide, a disaccharide, glucose, sucrose, fructose, leucrose, corn syrup, high fructose corn syrup, isomerized sugar, maltose, trehalose, panose, raffinose, cellobiose, isomaltose, honey, maple sugar, a fruit-derived sweetener, sorbitol, maltitol, isomaltitol, lactose, nigerose, kojibiose, xylitol, erythritol, dihydrochalcone, stevioside, α-glycosyl stevioside, acesulfame potassium, alitame, neotame, glycyrrhizin, thaumantin, sucralose, L-aspartyl-L-phenylalanine methyl ester, saccharine, maltodextrin, starch, potato starch, tapioca starch, dextran, soluble corn fiber, a resistant maltodextrin, a branched maltodextrin, inulin, polydextrose, a fructooligosaccharide, a galactooligosaccharide, a xylooligosaccharide, an arabinoxylooligosaccharide, a nigerooligosaccharide, a gentiooligosaccharide, hemicellulose, fructose oligomer syrup, an isomaltooligosaccharide, a filler, an excipient, a binder, or any combination thereof.

5 . A food product comprising the soluble α-glucan fiber composition of claim 1 or the carbohydrate composition of claim 3 or 4 .

6 . A method to produce a soluble α-glucan fiber composition comprising:
a. providing a set of reaction components comprising:
i. a maltodextrin substrate;
ii. at least one polypeptide having dextrin dextranase activity (E.C. 2.4.1.2);
iii. at least one polypeptide having endodextranase activity (E.C. 3.2.1.11) capable of endohydrolyzing glucan polymers having one or more α-(1,6) glycosidic linkages; and

b. combining the set of reaction components under suitable aqueous reaction conditions in a single reaction system whereby a product comprising a soluble α-glucan fiber composition is produced; and
c. optionally isolating the soluble α-glucan fiber composition from the product of step (b).

7 . The method of claim 6 further comprising step (d) concentrating the soluble α-glucan fiber composition.

8 . The method of claim 6 wherein combining the set of reaction components under suitable aqueous reaction conditions comprises combining the set of reaction components within a food product.

9 . The method of claim 6 wherein said at least one polypeptide having dextrin dextranase activity comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2.

10 . A method to make a blended carbohydrate composition comprising combining the soluble α-glucan fiber composition of claim 1 with: a monosaccharide, a disaccharide, glucose, sucrose, fructose, leucrose, corn syrup, high fructose corn syrup, isomerized sugar, maltose, trehalose, panose, raffinose, cellobiose, isomaltose, honey, maple sugar, a fruit-derived sweetener, sorbitol, maltitol, isomaltitol, lactose, nigerose, kojibiose, xylitol, erythritol, dihydrochalcone, stevioside, α-glycosyl stevioside, acesulfame potassium, alitame, neotame, glycyrrhizin, thaumantin, sucralose, L-aspartyl-L-phenylalanine methyl ester, saccharine, maltodextrin, starch, potato starch, tapioca starch, dextran, soluble corn fiber, a resistant maltodextrin, a branched maltodextrin, inulin, polydextrose, a fructooligosaccharide, a galactooligosaccharide, a xylooligosaccharide, an arabinoxylooligosaccharide, a nigerooligosaccharide, a gentiooligosaccharide, hemicellulose, fructose oligomer syrup, an isomaltooligosaccharide, a filler, an excipient, a binder, or any combination thereof.

11 . A method to reduce the glycemic index of a food or beverage comprising incorporating into a food or beverage the soluble α-glucan fiber composition of claim 1 whereby the glycemic index of a food or beverage is reduced.

12 . A method of inhibiting the elevation of blood-sugar level, lowering lipids, treating constipation, or altering the fatty acid production in a mammal comprising a step of administering the soluble α-glucan fiber composition of claim 1 to the mammal.

13 . A cosmetic composition, a pharmaceutical composition or a low cariogenicity composition comprising the soluble α-glucan fiber composition of claim 1 .

14 . Use of the soluble α-glucan fiber composition of claim 1 in a food composition suitable for consumption by animals, including humans.

15 . A composition comprising 0.01 to 99 wt % (dry solids basis) of the soluble α-glucan fiber composition of claim 1 and: a synbiotic, a peptide, a peptide hydrolysate, a protein, a protein hydrolysate, a soy protein, a dairy protein, an amino acid, a polyol, a polyphenol, a vitamin, a mineral, an herbal, an herbal extract, a fatty acid, a polyunsaturated fatty acid (PUFAs), a phytosteroid, betaine, a carotenoid, a digestive enzyme, a probiotic organism or any combination thereof.

Assignees

• Du Pont

Inventors

• Cheng Qiong
• Dicosimo Robert
• Eliot Andrew C
• Ouwehand Arthur
• Roesch Brian Michael
• Rothman Steven Cary
• Ruebling-Jass Kristin
• You Zheng

Classifications

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P3/06

  Antihyperlipidemics · CPC title

• C07H3/06

  Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages · CPC title

• A61P1/10

  Laxatives · CPC title

• C12Y302/01011

  Dextranase (3.2.1.11) · CPC title