Systems and methods for electrochemical aspartate transaminase (ast) and alanine transaminase (alt) detection and quantification

Patent metadata

Field	Value
Publication number	US-2017183711-A1
Application number	US-201615392593-A
Country	US
Kind code	A1
Filing date	Dec 28, 2016
Priority date	Dec 28, 2015
Publication date	Jun 29, 2017
Grant date	—

Abstract

A system for electrochemically detecting AST and ALT includes a first sampler for producing pyruvate from ALT and a second sampler for producing pyruvate from AST. The system further includes an electrochemical test strip for receiving processed samples from the first and second samplers, the processed samples containing pyruvate. The system further includes a meter for reading the electrochemical test strip and indicating an amount of AST and ALT in the sample.

First claim

What is claimed as new and desired to be protected by Letters Patent of the United States is:

1 . A system for electrochemically detecting ALT, the system comprising:
a sampler for producing pyruvate from ALT; an electrochemical test strip for receiving a processed sample from the sampler, the processed sample containing pyruvate; and a meter for reading the electrochemical test strip and indicating an amount of ALT in the sample.

2 . The system of claim 1 , wherein the sampler contains L-Alanine+alpha-Ketoglutarate.

3 . The system of claim 2 , wherein the electrochemical test strip includes phosphate and ferricyanide.

4 . The system of claim 3 , wherein the electrochemical test strip further includes pyruvate oxidase.

5 . The system of claim 4 , wherein the sampler includes a heating element.

6 . The system of claim 4 , wherein the electrochemical test strip includes a heating element.

7 . The system of claim 5 , wherein the sampler includes a timer.

8 . The system of claim 5 , wherein the meter includes a timer.

9 . A system for electrochemically detecting AST, the system comprising:
a sampler for producing pyruvate from AST; an electrochemical test strip for receiving a processed sample from the sampler, the processed sample containing pyruvate; and a meter for reading the electrochemical test strip and indicating an amount of AST in the sample.

10 . The system of claim 9 , wherein the sampler contains L-Aspartic acid+alpha-Ketoglutarate.

11 . The system of claim 10 , wherein the sampler further contains oxaloacetate decarboxylase.

12 . The system of claim 11 , wherein the electrochemical test strip includes phosphate and ferricyanide.

13 . The system of claim 12 , wherein the electrochemical test strip further includes pyruvate oxidase.

14 . The system of claim 13 , wherein the sampler includes a heating element.

15 . The system of claim 13 , wherein the electrochemical test strip includes a heating element.

16 . The system of claim 14 , wherein the sampler includes a timer.

17 . The system of claim 14 , wherein the meter includes a timer.

18 . A system for electrochemically detecting AST and ALT, the system comprising:
a first sampler for producing pyruvate from ALT; a second sampler for producing pyruvate from AST; an electrochemical test strip for receiving processed samples from the first and second samplers, the processed samples containing pyruvate; and a meter for reading the electrochemical test strip and indicating an amount of AST and ALT in the sample.

19 . The system of claim 18 , wherein the first sampler contains L-Alanine+alpha-Ketoglutarate.

20 . The system of claim 19 , wherein the second sampler contains L-Aspartic acid+alpha-Ketoglutarate.

21 . The system of claim 20 , wherein the second sampler further contains oxaloacetate decarboxylase.

22 . The system of claim 21 , wherein the electrochemical test strip includes phosphate, pyruvate oxidase, and ferricyanide.

23 . A system for electrochemically detecting phosphate, the system comprising:
an electrochemical test strip for receiving a sample, the electrochemical test strip includes pyruvate, pyruvate oxidase, and ferricyanide; and a meter for reading the electrochemical test strip and indicating an amount of phosphate in the sample.

Assignees

• Polymer Technology Systems Inc

Inventors

• Hughes Gary L
• Patwardhan Aniruddha

Classifications

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• G01N27/3273

  Devices therefor, e.g. test element readers, circuitry (details not specific to biochemical electrodes G01N33/4875) · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• G01N27/3272

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• C12Y102/03003

  Pyruvate oxidase (1.2.3.3) · CPC title