Systems and methods for sample use maximization

Patent metadata

Field	Value
Publication number	US-9464981-B2
Application number	US-201414562066-A
Country	US
Kind code	B2
Filing date	Dec 5, 2014
Priority date	Jan 21, 2011
Publication date	Oct 11, 2016
Grant date	Oct 11, 2016

Abstract

The present invention provides systems, devices, and methods for point-of-care and/or distributed testing services. The methods and devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device can be modified to allow for more flexible and robust use with the disclosed methods for a variety of medical, laboratory, and other applications. The systems, devices, and methods of the present invention can allow for effective use of samples by improved sample preparation and analysis.

First claim

What is claimed is:

1. A method of detecting the presence or concentration of an analyte in a sample fluid contained in a container comprising:
(a) illuminating the container along a first region having a first path length to yield a first measurement of light intensity transmitted across the first path length;
(b) determining that the first measurement falls outside a predetermined dynamic range of transmitted light intensity;
(c) moving the sample fluid to another region in the same container having another path length;
(d) illuminating the same container along the another region to yield another measurement of light intensity transmitted across the another path length; and
(e) repeating steps (c) and (d) as needed on the same container until a measurement of light intensity falls within the predetermined dynamic range, thereby detecting the presence or concentration of the analyte.

2. The method of claim 1 , wherein measurement of the light intensity is obtained by imaging.

3. The method of claim 1 , wherein the sample is moved from a first region of the container having a first path length to a second region of the container having another path length by aspirating the sample.

4. The method of claim 3 wherein an end of the container is attached to a pipette which is configured to aspirate the sample.

5. The method of claim 3 wherein the sample is moved up or down the length of the container.

6. The method of claim 1 , wherein the container is a pipette tip.

7. The method of claim 1 , wherein the container is conically shaped.

8. The method of claim 1 , wherein the container has two open ends.

9. The method of claim 8 , wherein a first open end has a greater diameter than a second open end.

10. The method of claim 1 , wherein the container has a plurality of distinct widths to permit transmission of light along a plurality of varying path lengths.

11. The method of claim 1 , wherein the container volume is less than 100 microliters.

12. A method of measuring an analyte concentration in a sample fluid comprising:
(a) providing the sample contained in a container dimensioned with a plurality of distinct widths to permit transmission of light along a plurality of varying path lengths that correspond to the plurality of distinct widths;
(b) illuminating the container along at least one of the plurality of path lengths; and
(c) imaging the container to measure a first light intensity transmitted across said at least one of the plurality of path lengths, for the determination of the concentration of the analyte based on the measured first light intensity;
(d) imaging the same container to measure a second light intensity transmitted across another path length corresponding to another distinct width of the container;
(e) comparing said first light intensity and the second light intensity; and
(f) determining an analyte concentration based on said comparing step.

13. The method of claim 12 , further comprising selecting a desired detection path length by one or more of the following: (a) moving a light source relative to the sample, (b) moving a detector relative to the sample, or (c) moving the sample within the container relative to the light source.

14. The method of claim 12 , wherein the illumination is provided by a light source and the imaging is provided by a detector, wherein the light source and the detector are on opposing sides of the container.

15. The method of claim 12 , wherein the illumination is provided by a light source and the imaging is provided by a detector, wherein the light source and the detector are on the same side of the container.

16. The method of claim 12 , wherein the plurality of path lengths are orthogonal to the length of the container.

17. The method of claim 12 , wherein the container has a first open end and a second open end, wherein the first open end has a smaller width than the second open end.

18. The method of claim 17 , wherein the second open end is configured to connect to a fluid handling device.

19. The method of claim 2 , further comprising deconvoluting a line scan of the image and detecting the presence or concentration of an analyte.

Assignees

• Theranos Inc

Inventors

• Gibbons Ian
• Nugent Anthony J
• Delacruz Anthony
• Young Daniel L
• Holmes Elizabeth A
• Drake Andrew
• Kemp Timothy Michael
• Balwani Sunny
• Pangarkar Chinmay

Classifications

• G01N33/54326

  Magnetic particles · CPC title

• G01N21/0303 Primary

  Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence) · CPC title

• G01N2035/00495

  Centrifuges · CPC title

• B04B5/0421

  pivotably mounted · CPC title

• G01N33/56972

  White blood cells · CPC title