Polypeptides Having Mannanase Activity and Polynucleotides Encoding Same

Patent metadata

Field	Value
Publication number	US-2017183643-A1
Application number	US-201414916476-A
Country	US
Kind code	A1
Filing date	Sep 19, 2014
Priority date	Sep 19, 2013
Publication date	Jun 29, 2017
Grant date	—

Abstract

The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.

First claim

1 - 38 (canceled)

39 . A polypeptide having mannanase activity, selected from the group consisting of:
(a) a polypeptide having at least 60% sequence identity to SEQ ID NO: 3 or the mature polypeptide of SEQ ID NO: 2; (b) a polypeptide having at least 81% sequence identity to SEQ ID NO: 6 or the mature polypeptide of SEQ ID NO: 5; (c) a polypeptide encoded by a polynucleotide that hybridizes under very low, low, medium, medium-high, high, or very high stringency conditions with:
(i) the mature polypeptide coding sequence of SEQ ID NO: 1,
(ii) the cDNA sequence thereof, or
(iii) the full-length complement of (i) or (ii);
(d) a polypeptide encoded by a polynucleotide that hybridizes under medium, medium-high, high, or very high stringency conditions with:
(i) the mature polypeptide coding sequence of SEQ ID NO: 4,
(ii) the cDNA sequence thereof, or
(iii) the full-length complement of (i) or (ii);
(e) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof; (f) a polypeptide encoded by a polynucleotide having at least 81% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 4 or the cDNA sequence thereof; (g) a variant of SEQ ID NO: 3 or the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; (h) a variant of SEQ ID NO: 6 or the mature polypeptide of SEQ ID NO: 5 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions; and (i) a fragment of the polypeptide of (a), (b), (c), (d), (e), (f), (g) or (h) that has mannanase activity.

40 . The polypeptide of claim 39 , comprising or consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 6 or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 5.

41 . The polypeptide of claim 39 , wherein the mature polypeptide corresponds to amino acids 1 to 541 of SEQ ID NO: 2, amino acids 121 to 541 of SEQ ID NO: 2, or amino acids 1 to 526 of SEQ ID NO: 5.

42 . A composition comprising the polypeptide of claim 39 .

43 . The composition of claim 42 , further comprising one or more additional enzymes.

44 . The composition of claim 42 , further comprising one or more detergent components.

45 . The composition of claim 42 , further comprising at least one fat soluble vitamin and/or at least one water soluble vitamin and/or at least one trace mineral.

46 . A method for degrading mannan comprising applying a composition of claim 42 to the mannan.

47 . The method of claim 46 , wherein the mannan is on the surface of a textile or hard surface, such as dish wash.

48 . The method of claim 46 , wherein the mannan is used in fracturing of a subterranean formation perpetrated by a well bore.

49 . The method of claim 48 , wherein the mannan is a component in borehole filtercake.

50 . A method for producing a coffee extract, comprising the steps:
(a) providing roast and ground coffee beans; (b) adding to said coffee beans water and a polypeptide of claim 39 ; (c) incubating to make an aqueous coffee extract; and (d) separating the coffee extract from the extracted coffee beans.

51 . The method of claim 50 , wherein step (b) further comprises adding an enzyme having β-1,3-galactanase activity.

52 . A process for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of the polypeptide of claim 39 .

53 . A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of the polypeptide of claim 39 ; (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and (c) recovering the fermentation product from the fermentation.

54 . The process of claim 53 , wherein the cellulosic material is pretreated.

55 . The process of claim 52 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of cellulase, AA9 polypeptide, hemicellulase, esterase, expansin, ligninolytic enzyme, oxidoreductase, pectinase, protease, and swollenin.

56 . A process of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of the polypeptide of claim 39 .

57 . The process of claim 56 , wherein the cellulosic material is pretreated before saccharification.

58 . The process of claim 56 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of cellulase, AA9 polypeptide, hemicellulase, esterase, expansin, ligninolytic enzyme, oxidoreductase, pectinase, protease, and swollenin.

59 . A polynucleotide encoding the polypeptide of claim 39 .

60 . A nucleic acid construct or expression vector comprising the polynucleotide of claim 59 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

61 . A recombinant host cell comprising the polynucleotide of claim 59 operably linked to one or more control sequences that direct the production of the polypeptide.

62 . A method of producing a polypeptide, comprising:
(a) cultivating the host cell of claim 61 under conditions conductive for production of the polypeptide; and (b) recovering the polypeptide.

Assignees

• Novozymes As

Inventors

• Krogh Kristian Bertel Roemer M
• Spodsberg Nikolaj
• Gori Klaus
• Von Freiesleben Pernille

Classifications

• C12Y302/01078

  Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase · CPC title

• C12N9/48

  acting on peptide bonds (3.4) · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title