Beta-glucosidase I variants with improved properties
US-9447400-B2 · Sep 20, 2016 · US
Polypeptides having beta-mannanase activity and methods of use
US9879245B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9879245-B2 |
| Application number | US-201314648677-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 2, 2013 |
| Priority date | Dec 7, 2012 |
| Publication date | Jan 30, 2018 |
| Grant date | Jan 30, 2018 |
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Abstract
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The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
First claim
Opening claim text (preview).
We claim: 1. A recombinant nucleic acid which comprises a nucleic acid encoding a recombinant polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:3 and a heterologous nucleic acid, wherein the recombinant polypeptide has beta-mannanase activity, and wherein said nucleic acid encoding the recombinant polypeptide is operably linked to the heterologous nucleic acid. 2. The recombinant nucleic acid of claim 1 , wherein the heterologous nucleic acid encodes a signal peptide sequence selected from any one of SEQ ID NOs:13-41. 3. A host cell comprising the recombinant nucleic acid of claim 1 , wherein the host cell is a microorganism. 4. The host cell of claim 3 wherein the heterologous nucleic acid encodes a signal peptide sequence. 5. The host cell of claim 4 wherein the signal peptide sequence is selected from any one of SEQ ID NOs: 13-41. 6. The host cell of claim 3 wherein the host cell is a bacterial cell or a fungal cell. 7. The host cell of claim 3 wherein the host cell is Trichoderma reesei. 8. The recombinant nucleic acid of claim 1 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 2. 9. The recombinant nucleic acid of claim 1 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 3. 10. The host cell of claim 3 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 2. 11. The host cell of claim 3 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 3.
Assignees
Inventors
Classifications
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
Beta-mannosidase (3.2.1.25), i.e. mannanase · CPC title
Beta-mannosidase (3.2.1.25), i.e. mannanase · CPC title
- C12N9/2494Primary
Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase · CPC title
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- What does patent US9879245B2 cover?
- The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (…
- Who is the assignee on this patent?
- Danisco Us Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2494. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 30 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).