Psicose Epimerase and Psicose Production Method Using Same

Patent metadata

Field	Value
Publication number	US-2017101637-A1
Application number	US-201515128812-A
Country	US
Kind code	A1
Filing date	May 22, 2015
Priority date	May 28, 2014
Publication date	Apr 13, 2017
Grant date	—

Abstract

The present invention provides a novel psicose epimerase derived from Flavonifractor plautii and capable of converting fructose to psicose. The novel psicose epimerase according to the present invention possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose to psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present invention is advantageous in the industrial production of psicose, and it is expected that the psicose produced thereby can be usefully utilized in the functional sugar industry and also as materials for health food, medicine, cosmetics, and the like using the psicose.

First claim

1 - 6 . (canceled)

7 . A composition for producing psicose, the composition comprising a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1, a recombinant strain, a culture of the recombinant strain, or a lysate of the recombinant strain, wherein the recombinant strain is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide.

8 . The composition for producing psicose of claim 7 , wherein the psicose epimerase is derived from Flavonifractor plautii.

9 . The composition for producing psicose of claim 7 , wherein the polynucleotide consists of a base sequence of SEQ ID NO: 2.

10 . The composition for producing psicose of claim 7 , wherein the recombinant vector has a cleavage map of FIG. 1 .

11 . The composition for producing psicose of claim 7 , the composition further comprising: one or more kinds selected from the group consisting of manganese ions, nickel ions, and cobalt ions.

12 . A method for producing psicose, the method comprising:
reacting fructose with a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1, a recombinant strain, a culture of the recombinant strain, a lysate of the recombinant strain, or a composition including one or more thereof, wherein the recombinant strain is transformed by a polynucleotide coding the wherein the recombinant strain is transformed by a polynucleotide coding the recombinant vector including the polynucleotide.

13 . The method for producing psicose of claim 12 , wherein the psicose epimerase is derived from Flavonifractor plautii.

14 . The method for producing psicose of claim 12 , wherein the polynucleotide consists of a base sequence of SEQ ID NO: 2.

15 . The method for producing psicose of claim 12 , wherein the recombinant vector has a cleavage map of FIG. 1 .

16 . The method for producing psicose of claim 12 , wherein the composition further includes one or more kinds selected from the group consisting of manganese ions, nickel ions, and cobalt ions.

17 . The method for producing psicose of claim 12 , wherein a temperature of the reaction is 55 to 67° C. and a pH of the reaction is 6.5 to 8.

18 . The method for producing psicose of claim 12 , wherein the concentration of the fructose is 35 to 75% (w/w).

19 . The method for producing psicose of claim 12 , wherein the psicose epimerase or the recombinant strain is immobilized in a carrier.

20 . The method for producing psicose of claim 12 , wherein a host strain of the recombinant strain is a cytologically safe strain.

Assignees

• Daesang Corp

Inventors

• Kim Tae Gyun
• Kim Min Su
• Kim Tae Yong
• Song Eun Bum
• Oh Deok Kun

Classifications

• C12N9/90 Primary

  Isomerases (5.) · CPC title

• C12P19/24

  produced by the action of an isomerase, e.g. fructose · CPC title

• C12Y501/03

  acting on carbohydrates and derivatives (5.1.3) · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title