Cell assessment method, cell assessment device, and cell assessment program

Patent metadata

Field	Value
Publication number	US-2016349170-A1
Application number	US-201415037142-A
Country	US
Kind code	A1
Filing date	Oct 17, 2014
Priority date	Nov 19, 2013
Publication date	Dec 1, 2016
Grant date	—

Abstract

The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

First claim

1 . A cell assessment method, comprising:
an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

2 . The cell assessment method according to claim 1 , wherein the small cell clump is a dissociated small clump product of a pluripotent stem cell colony.

3 . The cell assessment method according to claim 2 , wherein the pluripotent stem cell is an iPS cell or an ES cell.

4 . The cell assessment method according to claim 1 , wherein the small cell clump is a single cell.

5 . The cell assessment method according to claim 4 , further comprising a second extraction step of extracting a single cell region within the acquired optical path image.

6 . A cell assessment device, comprising:
an acquisition means acquiring an optical path length image of a small cell clump, an extraction means extracting a cell nucleus region within the acquired optical path length image, a comparison means comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment means assessing whether or not a cell is a stem cell based on the comparison results.

7 . The cell assessment device according to claim 6 , further comprising a second extraction means extracting a single cell region within the acquired optical path length image.

8 . A cell assessment program arranged to make a computer function as
an acquisition means acquiring an optical path length image of a small cell clump, an extraction means extracting a cell nucleus region within the acquired optical path length image, a comparison means comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment means assessing whether or not a cell is a stem cell based on the comparison results.

9 . The program according to claim 8 , further arranged to make the computer function as
a second extraction means extracting a single cell region within the acquired optical path length image.

Assignees

• Univ Kyoto
• Hamamatsu Photonics Kk

Inventors

• Nakatsuji Norio
• Sugiyama Norikazu
• Mizuguchi Yoshinori
• Fukami Tadashi
• Yamada Hidenao
• Yamauchi Toyohiko
• KAKUNO Yumi

Classifications

• G01N2015/1006

  for cytology · CPC title

• G01N2015/1447

  Spatial selection · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• G01N33/5073 Primary

  Stem cells · CPC title

• G01N15/1434 Primary

  Optical arrangements · CPC title