Self-assembling cell aggregates and methods of making engineered tissue using the same

Patent metadata

Field	Value
Publication number	US-2016348067-A1
Application number	US-201615235422-A
Country	US
Kind code	A1
Filing date	Aug 12, 2016
Priority date	Feb 24, 2004
Publication date	Dec 1, 2016
Grant date	—

Abstract

A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.

First claim

What is claimed is:

1 . A method of producing a tissue construct, comprising depositing cell aggregates from a dispensing device, and allowing the cell aggregates to fuse.

2 . The method of claim 1 , wherein the cell aggregates are dispensed in a pattern.

3 . The method of claim 1 , wherein the cell aggregates are homocellular.

4 . The method of claim 1 , wherein the cell aggregates are heterocellular.

5 . The method of claim 1 , wherein the dispensing device is instructed by a control unit.

Assignees

• Univ Missouri
• Musc Found For Res Dev

Inventors

• Forgacs Gabor
• Jakab Karoly
• Neagu Adrian
• Mironov Vladimir

Classifications

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B29K2105/0035

  Medical or pharmaceutical agents · CPC title

• B29L2031/7532

  Artificial members, protheses · CPC title

• C12N5/0697

  Artificial constructs associating cells of different lineages, e.g. tissue equivalents (blood vessels C12N5/0691) · CPC title

• B29C64/393

  for controlling or regulating additive manufacturing processes · CPC title