Production of fatty acid derivatives

Patent metadata

Field	Value
Publication number	US-2016333325-A1
Application number	US-201514709232-A
Country	US
Kind code	A1
Filing date	May 11, 2015
Priority date	Sep 25, 2009
Publication date	Nov 17, 2016
Grant date	—

Abstract

Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.

First claim

1 - 15 . (canceled)

16 . A genetically engineered microorganism for the production of a fatty ester, comprising an exogenous gene encoding an ester synthase (EC 2.3.1.75) polypeptide that has at least 90% sequence identity to SEQ ID NO: 18 and has an amino acid substitution at a residue corresponding to glycine 395, wherein said ester synthase polypeptide has an increased enzymatic activity relative to that of SEQ ID NO: 18, and wherein the microorganism produces an increased level of a fatty ester relative to a wild-type microorganism.

17 . The genetically engineered microorganism of claim 16 ,
comprising an exogenous control sequence stably incorporated into the genomic DNA of the microorganism upstream of the gene encoding the ester synthase polypeptide.

18 . The genetically engineered microorganism of claim 16 , wherein the microorganism is further genetically engineered to express, relative to a wild type microorganism, a decreased level of at least one gene encoding a fatty acid degradation enzyme.

19 . The genetically engineered microorganism of claim 16 , wherein the microorganism is further genetically engineered to express, relative to a wild type microorganism, a decreased level of at least one gene encoding a thioesterase.

20 . The genetically engineered microorganism of claim 16 , wherein the microorganism is further genetically engineered to express, relative to a wild type microorganism, a decreased level of at least one gene encoding an acyl-CoA dehydrogenase.

21 . The genetically engineered microorganism of claim 16 , wherein the microorganism is further genetically engineered to express, relative to a wild type microogranism, a decreased level of a gene encoding an outer membrane protein receptor.

22 . The genetically engineered microorganism of claim 16 , wherein said microorganism is a Gram-negative or a Gram-positive bacterium.

23 - 51 . (canceled)

52 . The genetically engineered microorganism of claim 16 , wherein said microorganism is a bacterium from Escherichia coli.

53 . The genetically engineered microorganism of claim 16 , wherein said microorganism is a cyanobacterium.

54 . The genetically engineered microorganism of claim 16 , wherein said exogenous gene encoding the ester synthase is derived from Acidobacteria bacterium, Acidothermus cellulolyticus, Acinetobacter baumannii, Acinetobacter baylyi, Acinetobacter sp., Acinetobacter baumannii, Acinetobacter sp., Aeromonas hydrophila, Aeromonas salmonicida, Alcaligenes europhus, Alcanivorax borkumensis, Alcanivorax jadensis, Alteromonas macleodii, Anaeromyxobacter dehalogenans, Anaeromyxobacter, Anaeromyxobacter sp., Arabidopsis thaliana, Bradyrhizobiurn japonicum, Cryptococcus curvatus, Erythrobacter litoralis, Erythrobacter sp., Frankia sp., Fundibacter jadensis, gamma proteobacterium, Hahella chejuensis, Homo sapiens, Janibacter sp., Limnobacter sp., marine gamma proteobacterium, Marinobacter algicola, Marinobacter aquaeolei, Marinobacter hydrocarbinoclasticus, Marinobacter sp., Methylibiurn petroleiphilum, Microscilla marina, Moritella sp., Mortierella alpina, Mus musculus, Mycobacterium abscessus, Mycobacterium avium, Mycobacterium avium, Mycobacterium Bovis, Mycobacterium gilvum, Mycobacterium leprae, Mycobacterium marinum, Mycobacterium smegmatis, Mycobacterium sp., Mycobacterium tuberculosis, Mycobacterium ulcerans, Mycobacterium vanbaalenii, Myxococcus xanthus, Natronomonas pharaonis, Nocardia farcinica, Nocardioides sp., Photobacterium profundum, Plesiocystis pacifica, Polaromonas naphthalenivorans, Polaromonas sp., Pseudomonas aeruginosa, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychrobacter sp., Reinekea sp., Rhodococcus opacus, Rhodoferax ferrireducens, Rhodococcus sp., Rhodoferax ferrireducens, Roseiflexus sp., Roseiflexus castenholzii, Saccharomyces cerevisiae, Saccharopolyspora erythraea, Salinibacter ruber, Simmodsia chinensis, Solibacter usitatus, Sphingopyxis alaskensis, Stigmatella aurantiaca, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces griseus, Tenacibaculum sp., or Ustilago maydis.

55 . The genetically engineered microorganism of claim 16 , wherein said fatty ester is a fatty acid ethyl ester (FAEE).

56 . The genetically engineered microorganism of claim 16 , wherein said fatty ester is a fatty acid methyl ester (FAME).

57 . The genetically engineered microorganism of claim 16 , wherein said fatty ester comprises a composition of a fatty acid ethyl ester (FAEE) and a fatty acid methyl ester (FAME).

56 . The genetically engineered microorganism of claim 16 , wherein said fatty ester is an ester of a long-chain alcohol.

57 . The genetically engineered microorganism of claim 16 , wherein said glycine 395 is replaced with a basic amino acid.

58 . The genetically engineered microorganism of claim 57 , wherein said glycine 395 is replaced with a lysine residue or an arginine residue.

Assignees

• Reg Life Sciences Llc

Inventors

• Gaertner Alfred

Classifications

• C10L1/19

  Esters {ester radical containing compounds; ester ethers; carbonic acid esters (C10L1/1802, C10L1/1805, C10L1/1808, C10L1/1811, C10L1/1814, C10L1/1817 take precedence)} · CPC title

• C10L1/026

  for compression ignition · CPC title

• C11C3/10

  Ester interchange · CPC title

• C12N9/1029 Primary

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12Y103/99003

  Acyl-CoA dehydrogenase (1.3.99.3) (C12Y103/08007, C12Y103/08008 and C12Y103/08009 take precedence) · CPC title