Thermolysin for easy-cleaning of insect body stains

Patent metadata

Field	Value
Publication number	US-2016304816-A1
Application number	US-201615193242-A
Country	US
Kind code	A1
Filing date	Jun 27, 2016
Priority date	Jun 21, 2010
Publication date	Oct 20, 2016
Grant date	—

Abstract

A substrate or coating is provided that includes a protease with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein an inventive substrate or coating including a protease is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from the substrate or said coating.

First claim

1 . A method of facilitating the removal of a biological stain on a substrate or a coating comprising:
providing a liquid coating material comprising an ammonia, an alcohol, or combinations thereof; associating a thermolysin-like protease with said coating material to form a liquid bioactive coating material such that said protease is capable of enzymatically degrading a component of a biological stain.

2 . The method of claim 1 wherein said liquid coating material includes said protease at a specific activity in excess of 20,000 U/g.

3 . The method of claim 1 wherein the pH of the liquid coating material is greater than 5.0.

4 . The method of claim 1 wherein said protease is in solution in said liquid coating material.

5 . The method of claim 1 wherein said liquid coating material comprises an alcohol, said alcohol present at less than 0.8% by weight.

6 . The method of claim 1 wherein said liquid coating material comprises ammonia, or a derivative of ammonia.

7 . The method of claim 1 further comprising applying said bioactive coating material to a biological stain, thereby promoting removal of said stain.

8 . The method of claim 7 wherein said biological stain is an insect stain.

9 . The method of claim 7 wherein said biological stain is present on a substrate.

10 . The method of claim 9 wherein said substrate is glass.

11 . A composition for facilitating biological stain removal comprising:
a liquid coating material comprising an ammonia, an alcohol, or combinations thereof; and a thermolysin-like protease, said protease capable of degrading a biological stain component and associated with said coating to form a liquid bioactive coating.

12 . The composition of claim 11 wherein said thermolysin-like protease is an active extracellular portion of bacterial neutral thermolysin-like-protease from Geobacillus stearothermophilus.

13 . The composition of claim 11 wherein said liquid bioactive coating material includes said protease at a specific activity in excess of 20,000 U/g.

14 . The composition of claim 11 wherein the pH of the liquid bioactive coating material is greater than 5.0.

15 . The composition of claim 11 wherein said protease is in solution in said liquid bioactive coating material.

16 . The composition of claim 11 wherein said liquid bioactive coating material comprises an alcohol, said alcohol present at less than 0.8% by weight.

17 . The composition of claim 11 wherein said liquid bioactive coating material comprises ammonia, or a derivative of ammonia.

Assignees

• Toyota Motor Eng & Mfg North America Inc
• Toyota Motor Corp

Inventors

• Wu Songtao
• Jia Hongfei
• Ishii Masahiko
• Zhang Minjuan

Classifications

• C11D3/2003

  Alcohols; Phenols · CPC title

• C11D3/38618 Primary

  Protease or amylase in liquid compositions only · CPC title

• C11D11/0035

  Chemistry & Metallurgy · mapped topic

• C11D3/04

  Water-soluble compounds · CPC title

• C11D7/04

  Water-soluble compounds · CPC title