Laser scanning observation device and laser scanning method

Patent metadata

Field	Value
Publication number	US-2016299170-A1
Application number	US-201414777535-A
Country	US
Kind code	A1
Filing date	Mar 28, 2014
Priority date	Mar 29, 2013
Publication date	Oct 13, 2016
Grant date	—

Abstract

Provided is a laser scanning observation device including: a window unit provided in a partial area of a casing and configured to be in contact with or close to an observation target; an objective lens configured to collect laser light on the observation target through the window unit; an optical path changing element configured to change a direction of travel of the laser light guided within the casing toward the window unit; an astigmatism correction element provided in a front stage of the window unit and configured to correct astigmatism occurring upon the collection of the laser light on the observation target; and a rotation mechanism configured to allow at least the optical path changing element to rotate about a rotation axis perpendicular to a direction of incidence of the laser light on the window unit to scan the observation target with the laser light.

First claim

1 . A laser scanning observation device comprising:
a window unit provided in a partial area of a casing and configured to be in contact with or close to an observation target; an objective lens configured to collect laser light on the observation target through the window unit; an optical path changing element configured to change a direction of travel of the laser light guided within the casing toward the window unit; an astigmatism correction element provided in a front stage of the window unit and configured to correct astigmatism occurring upon the collection of the laser light on the observation target; and a rotation mechanism configured to allow at least the optical path changing element to rotate about a rotation axis perpendicular to a direction of incidence of the laser light on the window unit to scan the observation target with the laser light, wherein the astigmatism correction element corrects astigmatism by an amount of correction corresponding to variation in the astigmatism caused by a change in depth of observation, the depth of observation being a measure of depth at a position where the laser light is collected on the observation target.

2 . The laser scanning observation device according to claim 1 ,
wherein the astigmatism correction element includes a lens having an at least two-sided cylindrical surface or toroidal surface through which the laser light passes, the astigmatism correction element being configured to rotate together with the optical path changing element by the rotation mechanism.

3 . The laser scanning observation device according to claim 2 ,
wherein the astigmatism correction element is a meniscus lens having a cylindrical surface formed on both surfaces.

4 . The laser scanning observation device according to claim 1 ,
wherein the astigmatism correction element is an optical member including a driving element configured to dynamically change the amount of correction for astigmatism depending on the change in the depth of observation.

5 . The laser scanning observation device according to claim 1 , further comprising:
a translational movement mechanism configured to allow at least the optical path changing element to move translationally in a direction of the rotation axis to scan the observation target with the laser light in the rotation axis direction.

6 . The laser scanning observation device according to claim 1 , further comprising:
a depth-of-observation adjusting mechanism configured to change the depth of observation to scan the observation target with the laser light in a depth direction.

7 . The laser scanning observation device according to claim 6 ,
wherein the depth-of-observation adjusting mechanism includes a collimator lens and a movement mechanism, the collimator lens being configured to collimate the laser light into a substantially parallel beam of light and to guide the collimated light to the optical path changing element and the astigmatism correction element, the movement mechanism being configured to move the collimator lens in a direction of an optical axis.

8 . The laser scanning observation device according to claim 1 ,
wherein the laser scanning observation device detects fluorescent light occurring by irradiating the observation target with the laser light as returning light to acquire information relating to the observation target, and wherein the laser scanning observation device further includes a chromatic aberration correction element configured to correct chromatic aberration caused by a difference in wavelengths between the laser light and the fluorescent light.

9 . The laser scanning observation device according to claim 8 ,
wherein the chromatic aberration correction element is a cemented lens configured to function as a parallel flat plate for light having a wavelength band corresponding to the laser light and to function as a concave lens for light having a wavelength band corresponding to the fluorescent light.

10 . The laser scanning observation device according to claim 1 ,
wherein the optical path changing element is configured to allow a pencil of the laser light to be incident on the optical path changing element, and wherein the objective lens collects the pencil of the laser light at a plurality of different spots of the observation target.

11 . The laser scanning observation device according to claim 10 ,
wherein the pencil of the laser light is configured to include the laser light modulated to a plurality of different states.

12 . The laser scanning observation device according to claim 10 ,
wherein the pencil of the laser light is guided into the casing through a plurality of optical fibers.

13 . The laser scanning observation device according to claim 10 ,
wherein the pencil of the laser light is guided into the casing through a multi-core optical fiber including a plurality of cores.

14 . The laser scanning observation device according to claim 1 , further comprising:
a polarization modulation element provided in a front stage of the optical path changing element and configured to change a polarization direction of the laser light incident on the optical path changing element, wherein the optical path changing element is a polarization beam splitter configured to change an optical path of the laser light having a predetermined polarization direction, and wherein the polarization beam splitter changes a direction of travel of the laser light of which a polarization direction is changed by the polarization modulation element toward the window unit depending on the polarization direction of the laser light.

15 . The laser scanning observation device according to claim 1 , further comprising:
an optical path branching element provided in a front stage of the optical path changing element and configured to allow the laser light incident on the optical path changing element to be branched into a plurality of optical paths, wherein the astigmatism correction element, the optical path changing element, and the objective lens are provided for each of the plurality of optical paths, and wherein the optical path changing element changes each direction of travel of the laser light branched by the optical path branching element to a plurality of directions perpendicular to a direction of the rotation axis.

16 . The laser scanning observation device according to claim 1 ,
wherein the laser scanning observation device is provided with a housing configured to accommodate at least a plurality of the optical path changing elements and to rotate together with the plurality of optical path changing elements, wherein the housing includes an incident window unit formed on a wall of the housing on which the laser light is incident and configured to allow the laser light to be incident on each of the plurality of optical path changing elements, wherein the astigmatism correction element and the objective lens are provided for each of a plurality of the incident window units, wherein the laser light is guided within the casing in a state where an optical axis of the laser light is maintained at a predetermined position with respect to the casing and the laser light is sequentially applied to the plurality of incident window units with a rotation of the housing, and wherein the laser light incident through the incident window unit corresponding to a position to be irradiated with the laser light is guided to the window unit by the optical path changing element.

17 . The laser scanning observation device according to claim 1 ,
wherein the cas

Assignees

• Sony Corp

Inventors

• Ito Terumasa
• Fukumoto Atsushi
• Maeda Fumisada
• Chubachi Hideya
• Hirono Yu

Classifications

• G02B23/2469

  using optical fibres · CPC title

• A61B1/00177

  for 90 degrees side-viewing · CPC title

• G02B23/2423

  of the distal end · CPC title

• A61B1/00172

  with means for scanning · CPC title

• G02B21/0072

  details concerning resolution or correction, including general design of CSOM objectives · CPC title